MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4247)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4287 897.9153 1793.8160 1793.8505 -19.2 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
MNDLTSDLPYEDILR
3934 584.7990 1167.5834 1167.5706 11.0 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
RSGYMVNTPK + Oxidation (M)
3914 572.2892 1142.5638 1142.5502 11.9 1 4 0.94 +1Score > 22 indicates identity
Score > 16 indicates homology
QRAGTPEMPR + Deamidated (NQ)
4375 749.0685 2244.1837 2244.2188 -15.6 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
KLILEADVVLDGYRPGVMEK
3550 387.7417 773.4688 773.4647 5.37 1 4 0.45 +1Score > 20 indicates identity
Score > 13 indicates homology
KVDIATK
4371 742.7155 2225.1247 2225.1427 -8.08 0 4 0.45 +1Score > 23 indicates identity
Score > 13 indicates homology
LNELQASDVSLRPDPEIISK + 2 Deamidated (NQ)
4117 695.8842 1389.7538 1389.7728 -13.7 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
GNASVYGNVIKLR
4391 773.7120 2318.1142 2318.1430 -12.4 1 4 1 +1Score > 24 indicates identity
Score > 16 indicates homology
AQLPELSYYQAVGKSAETPHK + 2 Deamidated (NQ)
4201 784.9163 1567.8180 1567.8392 -13.5 1 4 0.44 +1Score > 23 indicates identity
Score > 13 indicates homology
SRLQTAPVPMPDLK + Oxidation (M)
3989 414.9210 1241.7412 1241.7166 19.8 0 4 1.1 +1Score > 16 indicates identity IPAMGATSLIIR
4013 633.8184 1265.6222 1265.6074 11.7 1 4 0.75 +1Score > 24 indicates identity
Score > 15 indicates homology
KVFGAMDDLDR
3415 321.7385 641.4624 641.4588 5.69 1 4 0.62 +1Score > 14 indicates identity KLLLR
4286 597.6679 1789.9819 1789.9951 -7.40 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
WQLAPPTTPRPLTRR + Deamidated (NQ)
4350 696.3696 2086.0870 2086.0517 16.9 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
MLSQIDEPYPKDIERPR
4319 635.0038 1901.9896 1902.0139 -12.8 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
SGVKVVTIYAFSIENFK + Deamidated (NQ)
4288 599.3322 1794.9748 1794.9529 12.2 1 4 2.1 +1Score > 19 indicates identity WGNLAEVNAVRFAHLV
4381 752.4169 2254.2289 2254.1966 14.3 0 4 1.9 +1Score > 19 indicates identity QVDVLIVGAGPAGLMAALWMAR + Oxidation (M)
3740 319.1786 954.5140 954.5148 -0.82 1 4 0.57 +1Score > 21 indicates identity
Score > 14 indicates homology
NLYRHPR
3962 602.2956 1202.5766 1202.5567 16.5 0 4 0.88 +1Score > 24 indicates identity
Score > 16 indicates homology
DGNVNIWDLR + 2 Deamidated (NQ)
3780 502.2673 1002.5200 1002.5345 -14.5 1 4 1 +1Score > 25 indicates identity
Score > 16 indicates homology
AEKVAQETK
3922 577.2993 1152.5840 1152.5788 4.53 0 4 0.82 +1Score > 22 indicates identity
Score > 15 indicates homology
FRPQSEHPR
3788 338.1814 1011.5224 1011.5025 19.6 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
AFEDYVLR
4341 670.6865 2009.0377 2009.0694 -15.8 1 4 0.51 +1Score > 23 indicates identity
Score > 13 indicates homology
DIISNISNTPPVRFHTAK
3525 372.7183 743.4220 743.4330 -14.7 0 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
ALQWVK
4254 556.9469 1667.8189 1667.8114 4.45 1 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
SGAVPQRDLDELPNR + 2 Deamidated (NQ)
4101 457.9234 1370.7484 1370.7704 -16.0 1 3 0.69 +1Score > 22 indicates identity
Score > 14 indicates homology
GIKSAAMVLAASPR
3835 527.7520 1053.4894 1053.4801 8.90 0 3 0.85 +1Score > 21 indicates identity
Score > 15 indicates homology
AEVAFMNEK + Oxidation (M)
3711 310.5143 928.5211 928.5164 5.01 0 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
QVVPLSMR
3631 428.7688 855.5230 855.5290 -6.97 1 3 0.91 +1Score > 20 indicates identity
Score > 15 indicates homology
IAEIVRR
4360 1063.0547 2124.0948 2124.0959 -0.48 1 3 0.55 +1Score > 23 indicates identity
Score > 13 indicates homology
SVQRIMDVLAAMIQEFLK + Deamidated (NQ); 2 Oxidation (M)
4285 596.6326 1786.8760 1786.8957 -11.0 0 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
IASYVINSMSSAMILR + 2 Oxidation (M)
3613 418.7325 835.4504 835.4439 7.79 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
VAELYNK
3980 615.3502 1228.6858 1228.6775 6.78 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ALIDLNSTNLR
3905 566.3151 1130.6156 1130.5931 19.9 1 3 0.97 +1Score > 24 indicates identity
Score > 16 indicates homology
KDSDNKPSIK
3634 430.2158 858.4170 858.4195 -2.87 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
NAAAVQQR + 2 Deamidated (NQ)
4025 429.2299 1284.6679 1284.6534 11.2 1 3 0.78 +1Score > 23 indicates identity
Score > 15 indicates homology
QIAQQDAERAR
4112 463.5784 1387.7134 1387.6884 18.0 1 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
LHPDFKDVNFR + Deamidated (NQ)
4251 554.9335 1661.7787 1661.7719 4.08 0 3 0.78 +1Score > 23 indicates identity
Score > 15 indicates homology
NMEGAHLVTIEEFR + Deamidated (NQ); Oxidation (M)
4361 716.7101 2147.1085 2147.1045 1.87 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
IVCNNDQQASIFLQQKLK + Deamidated (NQ)
3439 329.6889 657.3632 657.3558 11.3 1 3 2.3 +1Score > 19 indicates identity SPNGRK
3599 411.7292 821.4438 821.4548 -13.3 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
RGLWYK
4448 1130.5560 3388.6462 3388.6166 8.72 0 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
ALSLADNSTALLVEYSEEVPVMLSNFGMSNR + 2 Oxidation (M)
4332 654.0128 1959.0166 1958.9949 11.1 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
IVLDTDDPAFGGLNRNLK + 2 Deamidated (NQ)
3642 435.7764 869.5382 869.5334 5.54 0 3 1.6 +1Score > 18 indicates identity VQLELLR
3850 537.2859 1072.5572 1072.5625 -4.90 1 3 0.63 +1Score > 25 indicates identity
Score > 14 indicates homology
LGNRSDNAVK
3860 542.8431 1083.6716 1083.6651 6.00 1 3 0.79 +1Score > 15 indicates identity LAELIEKLR
3593 409.7231 817.4316 817.4480 -20.0 1 3 1 +1Score > 25 indicates identity
Score > 16 indicates homology
LGNRVMK + Deamidated (NQ)
3925 579.3209 1156.6272 1156.6451 -15.5 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
ALANALESIVR + Deamidated (NQ)
3517 368.2070 734.3994 734.4075 -11.0 1 3 0.59 +1Score > 22 indicates identity
Score > 13 indicates homology
KLFGDR
4121 470.9056 1409.6950 1409.6986 -2.58 1 3 0.67 +1Score > 24 indicates identity
Score > 14 indicates homology
IMQPSHARWER
3556 393.2370 784.4594 784.4443 19.3 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
KPAGLGDK
4314 943.9979 1885.9812 1885.9720 4.91 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
IYGNAIQMLYGKISSGR + Oxidation (M)
4041 653.7731 1305.5316 1305.5507 -14.6 0 3 1.2 +1Score > 16 indicates identity GSTLDDPMPNSR + Deamidated (NQ); Oxidation (M)
4087 341.9443 1363.7481 1363.7401 5.88 1 3 0.7 +1Score > 20 indicates identity
Score > 14 indicates homology
QFLFRFEHIK
4228 803.9052 1605.7958 1605.8263 -19.0 1 3 0.79 +1Score > 24 indicates identity
Score > 14 indicates homology
VSAREIDAYWLQR
3779 502.2348 1002.4550 1002.4553 -0.21 0 3 0.76 +1Score > 21 indicates identity
Score > 14 indicates homology
MHNLSTQR + Deamidated (NQ); Oxidation (M)
3977 611.8420 1221.6694 1221.6903 -17.1 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QAFMRSLILK + Oxidation (M)
3759 489.2776 976.5406 976.5229 18.2 0 3 0.71 +1Score > 22 indicates identity
Score > 14 indicates homology
FEINAALAK + Deamidated (NQ)
4416 854.7804 2561.3194 2561.3208 -0.56 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
SAKIIAMMVVGFCVLAAFVAYER + Oxidation (M)
3636 432.2300 862.4454 862.4548 -10.9 0 3 0.56 +1Score > 23 indicates identity
Score > 13 indicates homology
QKPQSFK + Deamidated (NQ)
3764 494.2617 986.5088 986.5145 -5.73 0 3 0.72 +1Score > 24 indicates identity
Score > 14 indicates homology
AQQSADIVR
4049 658.8536 1315.6926 1315.6884 3.19 1 3 0.64 +1Score > 24 indicates identity
Score > 13 indicates homology
KQYHTNTPTVK
4392 775.7183 2324.1331 2324.1358 -1.18 1 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
MGFLNIWSTGKVQNLANATQK + 4 Deamidated (NQ)
4182 758.4263 1514.8380 1514.8416 -2.36 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QLTLSDATLRIER
3790 338.1855 1011.5347 1011.5210 13.5 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
SLHARGESR
3483 350.2437 698.4728 698.4691 5.43 0 3 1.1 +1Score > 16 indicates identity LQVIVK
3937 586.3287 1170.6428 1170.6244 15.7 0 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
QLVEDIEGLR
3520 369.1965 736.3784 736.3755 3.96 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
SLIYNQ
3861 543.3170 1084.6194 1084.5989 19.0 1 3 0.99 +1Score > 22 indicates identity
Score > 15 indicates homology
LVRAVANGER + Deamidated (NQ)
4304 464.0099 1852.0105 1851.9843 14.2 1 3 0.66 +1Score > 20 indicates identity
Score > 13 indicates homology
GLNVPSILRIENSWPR + 2 Deamidated (NQ)
3428 325.1888 648.3630 648.3595 5.45 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
IVFDR
3656 442.7441 883.4736 883.4763 -3.01 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
QLEAVAPR + Deamidated (NQ)
3910 567.8022 1133.5898 1133.5717 16.0 0 2 1 +1Score > 24 indicates identity
Score > 15 indicates homology
IIFGDEVADR
3384 308.7122 615.4098 615.4068 5.00 1 2 0.93 +1Score > 20 indicates identity
Score > 15 indicates homology
KLLSR
4064 669.8525 1337.6904 1337.6714 14.2 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
SIVATIQGEKYE + Deamidated (NQ)
3647 437.7145 873.4144 873.4093 5.89 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
HGLDNYR
4386 571.5407 2282.1337 2282.1464 -5.56 1 2 1 +1Score > 24 indicates identity
Score > 15 indicates homology
GSITTQDFLNEATKIMDIIR + Deamidated (NQ); Oxidation (M)
3988 621.8776 1241.7406 1241.7166 19.4 1 2 1.5 +1Score > 16 indicates identity MPVVIARSLQK + Deamidated (NQ)
4247 827.9246 1653.8346 1653.8079 16.2 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
NAMNLIYSNRTMAR
4048 658.8349 1315.6552 1315.6619 -5.09 0 2 0.95 +1Score > 24 indicates identity
Score > 15 indicates homology
LQSTAQDVISPR + 2 Deamidated (NQ)
3371 301.6865 601.3584 601.3548 6.15 0 2 1 +1Score > 27 indicates identity
Score > 15 indicates homology
LTVNR
3826 523.2935 1044.5724 1044.5644 7.70 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
LFKFSFQK + Deamidated (NQ)
3919 576.2859 1150.5572 1150.5466 9.29 1 2 1 +1Score > 24 indicates identity
Score > 15 indicates homology
SQQSNTSLKR + 3 Deamidated (NQ)
3822 523.2825 1044.5504 1044.5451 5.13 1 2 0.88 +1Score > 24 indicates identity
Score > 14 indicates homology
EREQQLLK + 2 Deamidated (NQ)
3356 566.2635 565.2562 565.2530 5.72 0 2 2.8 +1Score > 19 indicates identity NTMGK + Oxidation (M)
3947 396.9018 1187.6836 1187.6914 -6.57 0 2 2.9 +1Score > 19 indicates identity LIPQLLNSYK
4394 791.4102 2371.2088 2371.2246 -6.66 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
LNKYVNPSLIMPLDGAWLPGR + 2 Deamidated (NQ); Oxidation (M)
3831 525.3425 1048.6704 1048.6505 19.0 1 2 0.62 +1Score > 13 indicates identity VSKPPLPRR
4045 656.4065 1310.7984 1310.7921 4.81 1 2 0.74 +1Score > 13 indicates identity GLLLEILGRQAK + Deamidated (NQ)
4397 597.5751 2386.2713 2386.2910 -8.24 1 2 0.99 +1Score > 21 indicates identity
Score > 15 indicates homology
HWRLIGWANIVAGNTLEPILN
3958 401.2205 1200.6397 1200.6363 2.79 1 2 1 +1Score > 24 indicates identity
Score > 15 indicates homology
KAPQRPEYGR
4249 831.3970 1660.7794 1660.7879 -5.08 0 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
NMEGAHLVTIEEFR + Oxidation (M)
3408 317.1946 632.3746 632.3857 -17.5 1 2 1.7 +1Score > 17 indicates identity SSLKAK
3746 322.1762 963.5068 963.4960 11.2 0 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology
QPPICPPR
4028 429.2445 1284.7117 1284.6939 13.9 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
QHSIGGFKVQGK
4132 714.3716 1426.7286 1426.7416 -9.05 1 2 0.9 +1Score > 23 indicates identity
Score > 14 indicates homology
GPSLKEIQEAEAR
3651 438.7313 875.4480 875.4535 -6.20 0 2 1 +1Score > 25 indicates identity
Score > 14 indicates homology
MLTSPTAR
3567 395.2366 788.4586 788.4545 5.32 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
IFREPK
3903 564.8328 1127.6510 1127.6299 18.8 0 2 0.78 +1Score > 21 indicates identity
Score > 13 indicates homology
STLLNNLPTR
4419 648.5792 2590.2877 2590.3247 -14.3 1 2 1 +1Score > 23 indicates identity
Score > 14 indicates homology
GQVLMIPALVTEDCYASQVARLR + Deamidated (NQ)
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