MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3268 491.2281 490.2208
3269 491.2286 490.2213
3270 493.0193 492.0120
3271 493.0194 492.0121
3272 493.0196 492.0123
3273 493.0197 492.0124
3274 493.0197 492.0124
3275 493.0198 492.0125
3276 493.0205 492.0132
3277 493.2436 492.2363
3278 493.2439 492.2366
3279 493.2440 492.2367
3280 493.2441 492.2368
3281 493.2441 492.2368
3282 493.2441 492.2368
3283 493.2443 492.2370
3284 493.2443 492.2370
3285 493.2447 492.2374
3286 493.2448 492.2375
3287 495.2227 494.2154
3288 495.2241 494.2168
3289 495.2242 494.2169
3290 495.2245 494.2172
3291 495.2594 494.2521
3292 495.2595 494.2522
3293 495.2596 494.2523
3294 495.2596 494.2523
3295 495.2597 494.2524
3296 495.2598 494.2525
3297 495.2599 494.2526
3298 495.2599 494.2526
3299 495.2600 494.2527
3300 497.1719 496.1646
3301 497.1721 496.1648
3302 497.2388 496.2315
3303 497.2389 496.2316
3304 497.2397 496.2324
3305 497.2748 496.2675
3306 497.2753 496.2680
3307 499.1511 498.1438
3308 500.3588 499.3515
3309 502.1158 501.1085
3311 503.1895 502.1822
3313 507.2231 506.2158
3314 507.2234 506.2161
3315 507.2236 506.2163
3317 509.2388 508.2315
3318 509.2390 508.2317
3319 509.2391 508.2318
3320 509.2393 508.2320
3321 509.2393 508.2320
3322 509.2395 508.2322
3323 511.2544 510.2471
3324 511.2553 510.2480
3325 511.2555 510.2482
3326 511.2556 510.2483
3327 513.2711 512.2638
3328 513.2814 512.2741
3329 515.3326 514.3253
3330 517.1620 516.1547
3331 517.2998 516.2925
3332 524.2862 523.2789
3333 525.2334 524.2261
3334 525.2711 524.2638
3335 525.2914 524.2841
3336 527.1823 526.1750
3337 527.1829 526.1756
3338 529.2754 528.2681
3339 529.3128 528.3055
3342 533.1552 532.1479
3344 537.2327 536.2254
3346 545.3319 544.3246
3347 547.1707 546.1634
3348 550.2678 549.2605
3349 557.8249 556.8176
3350 558.2937 557.2864
3357 567.2446 566.2373
3358 567.2448 566.2375
3359 569.3176 568.3103
3360 569.3183 568.3110
3362 574.3218 573.3145
3363 577.2789 576.2716
3365 581.2604 580.2531
3366 581.2610 580.2537
3368 596.9669 595.9596
3374 302.6692 603.3238
3375 305.6646 609.3146
3376 306.1990 610.3834
3378 307.1140 612.2134
3379 613.2857 612.2784
3380 613.3444 612.3371
3385 309.6594 617.3042
3386 618.3120 617.3047
3387 309.6732 617.3318
3388 310.1192 618.2238
3389 311.6833 621.3520
3391 313.1646 624.3146
3392 314.1511 626.2876
3400 315.1297 628.2448
3401 315.1458 628.2770
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