MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2768 425.1808 424.1735
2769 425.1809 424.1736
2770 425.1809 424.1736
2771 425.1810 424.1737
2772 425.1810 424.1737
2773 425.1811 424.1738
2774 425.1814 424.1741
2775 425.2157 424.2084
2776 425.2163 424.2090
2777 425.2168 424.2095
2778 425.2170 424.2097
2779 425.2171 424.2098
2780 425.2173 424.2100
2781 425.2175 424.2102
2782 425.2175 424.2102
2783 425.2175 424.2102
2784 425.2176 424.2103
2785 425.2179 424.2106
2786 425.2180 424.2107
2787 425.2182 424.2109
2788 425.2343 424.2270
2789 425.2351 424.2278
2790 425.2351 424.2278
2791 425.2351 424.2278
2792 425.2354 424.2281
2793 425.2887 424.2814
2794 425.2889 424.2816
2795 425.2892 424.2819
2796 425.2894 424.2821
2797 425.2894 424.2821
2798 426.2375 425.2302
2799 427.1301 426.1228
2800 427.1309 426.1236
2801 427.1312 426.1239
2802 427.1674 426.1601
2803 427.1956 426.1883
2804 427.1964 426.1891
2805 427.1967 426.1894
2806 427.1968 426.1895
2807 427.1969 426.1896
2808 427.1969 426.1896
2809 427.1971 426.1898
2810 427.2325 426.2252
2811 427.2328 426.2255
2812 427.2328 426.2255
2813 427.2329 426.2256
2814 427.2332 426.2259
2815 427.2333 426.2260
2816 427.2333 426.2260
2817 427.2334 426.2261
2818 427.2336 426.2263
2819 427.2508 426.2435
2820 427.2508 426.2435
2821 427.2528 426.2455
2822 428.2535 427.2462
2823 428.2538 427.2465
2824 428.2542 427.2469
2825 428.2891 427.2818
2826 429.1081 428.1008
2827 429.1090 428.1017
2828 429.1463 428.1390
2829 429.1464 428.1391
2830 429.1474 428.1401
2831 429.1542 428.1469
2832 429.1828 428.1755
2833 430.0937 429.0864
2834 430.0944 429.0871
2835 430.2429 429.2356
2836 430.3000 429.2927
2837 430.3003 429.2930
2838 430.3012 429.2939
2839 431.0196 430.0123
2840 431.0874 430.0801
2841 431.1248 430.1175
2842 431.1263 430.1190
2843 433.1853 432.1780
2844 433.1856 432.1783
2845 433.1856 432.1783
2846 433.1859 432.1786
2847 433.1859 432.1786
2848 433.1859 432.1786
2849 433.1860 432.1787
2850 433.1861 432.1788
2851 433.1865 432.1792
2852 433.2221 432.2148
2853 433.2224 432.2151
2854 433.2225 432.2152
2855 433.2230 432.2157
2856 435.0117 434.0044
2857 435.1263 434.1190
2858 435.1656 434.1583
2859 435.2009 434.1936
2860 435.2010 434.1937
2861 435.2012 434.1939
2862 435.2012 434.1939
2863 435.2012 434.1939
2864 435.2012 434.1939
2865 435.2012 434.1939
2866 435.2013 434.1940
2867 435.2013 434.1940
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