MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 4247)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3399 314.7249 627.4352 627.4432 -12.6 1 7 0.42 +1Score > 16 indicates identity LVLKR
3381 307.6986 613.3826 613.3799 4.48 0 7 0.73 +1Score > 20 indicates identity
Score > 19 indicates homology
LPASVK
4034 431.9149 1292.7229 1292.7387 -12.2 1 7 0.87 +1Score > 19 indicates identity MALRLPPQVPR + Oxidation (M)
3619 422.2584 842.5022 842.5086 -7.55 1 7 0.37 +1Score > 23 indicates identity
Score > 16 indicates homology
QRISLAR
3513 365.2364 728.4582 728.4585 -0.32 0 7 0.25 +1Score > 22 indicates identity
Score > 14 indicates homology
IGLWIK
3372 302.1970 602.3794 602.3751 7.16 1 7 0.59 +1Score > 18 indicates identity TLKNK
3797 509.7643 1017.5140 1017.5243 -10.1 0 7 0.8 +1Score > 25 indicates identity
Score > 19 indicates homology
TLASPPYNR
3683 453.7222 905.4298 905.4355 -6.24 1 7 0.68 +1Score > 24 indicates identity
Score > 18 indicates homology
NKYDPNR
3752 487.2741 972.5336 972.5505 -17.3 0 7 1 +1Score > 24 indicates identity
Score > 20 indicates homology
TPLHHAVAK
4018 640.3116 1278.6086 1278.5993 7.32 0 7 1 +1Score > 24 indicates identity
Score > 20 indicates homology
QADFQTYLHR + Deamidated (NQ)
3459 336.7128 671.4110 671.4078 4.80 1 7 0.92 +1Score > 23 indicates identity
Score > 19 indicates homology
ANKALR
3572 399.7783 797.5420 797.5487 -8.33 1 7 0.2 +1Score > 13 indicates identity LVGLIRK
4014 423.9043 1268.6911 1268.6989 -6.20 1 7 0.23 +1Score > 20 indicates identity
Score > 13 indicates homology
IPGDGILSRWR
3768 496.7772 991.5398 991.5484 -8.67 1 7 0.27 +1Score > 22 indicates identity
Score > 14 indicates homology
LSSGRMVVK + Oxidation (M)
3915 572.7761 1143.5376 1143.5455 -6.83 1 7 1 +1Score > 21 indicates identity
Score > 20 indicates homology
LSNTEHMRR + Deamidated (NQ)
3562 394.7134 787.4122 787.4076 5.93 0 7 0.96 +1Score > 25 indicates identity
Score > 19 indicates homology
GGQVVEAK + Deamidated (NQ)
3754 487.7773 973.5400 973.5280 12.4 1 7 0.52 +1Score > 22 indicates identity
Score > 17 indicates homology
VFLRHMR + Oxidation (M)
3828 523.8069 1045.5992 1045.5808 17.6 0 7 0.9 +1Score > 20 indicates identity
Score > 19 indicates homology
FLPGLLSGDK
3632 429.7395 857.4644 857.4719 -8.68 1 7 0.85 +1Score > 24 indicates identity
Score > 19 indicates homology
ADIERVR
3444 330.1973 658.3800 658.3762 5.84 1 7 0.65 +1Score > 24 indicates identity
Score > 18 indicates homology
AEGVKR
4100 685.8972 1369.7798 1369.7929 -9.52 1 7 0.28 +1Score > 20 indicates identity
Score > 14 indicates homology
AIVGASLDVIKER
4252 556.6526 1666.9360 1666.9559 -12.0 1 7 0.81 +1Score > 18 indicates identity VAILFFRSTTIPFR
3448 330.6833 659.3520 659.3425 14.5 0 7 0.78 +1Score > 27 indicates identity
Score > 18 indicates homology
AMPVAR + Oxidation (M)
3769 496.7773 991.5400 991.5372 2.89 1 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology
EILKQMSK + Oxidation (M)
3804 513.7327 1025.4508 1025.4423 8.37 0 7 1 +1Score > 19 indicates identity YMSMTHTR
4009 421.8691 1262.5855 1262.5813 3.33 0 7 0.29 +1Score > 23 indicates identity
Score > 14 indicates homology
EGGDLVPGAMSSK + Oxidation (M)
3667 445.7624 889.5102 889.5021 9.12 0 7 1 +1Score > 20 indicates identity
Score > 19 indicates homology
GIPITYAR
3692 457.7755 913.5364 913.5484 -13.1 0 7 0.52 +1Score > 21 indicates identity
Score > 16 indicates homology
TIAQLLAAL + Deamidated (NQ)
4129 476.5830 1426.7272 1426.7304 -2.24 0 7 0.31 +1Score > 24 indicates identity
Score > 14 indicates homology
EGLDITLSDIHSK
3737 478.2374 954.4602 954.4705 -10.8 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology
ISGHMASPR
3449 330.7014 659.3882 659.3966 -12.7 0 7 0.96 +1Score > 22 indicates identity
Score > 19 indicates homology
LGLTTR
3497 358.7206 715.4266 715.4228 5.35 0 6 0.44 +1Score > 22 indicates identity
Score > 15 indicates homology
QSLLVR + Deamidated (NQ)
4096 684.8808 1367.7470 1367.7409 4.52 1 6 0.34 +1Score > 21 indicates identity
Score > 14 indicates homology
SPSALLQPGDRVK + Deamidated (NQ)
3944 592.7966 1183.5786 1183.5662 10.5 0 6 0.31 +1Score > 22 indicates identity
Score > 14 indicates homology
WAAQYGDVFK
3624 425.7271 849.4396 849.4344 6.12 1 6 0.56 +1Score > 23 indicates identity
Score > 16 indicates homology
LGFKDNR + Deamidated (NQ)
4116 464.2424 1389.7054 1389.7100 -3.31 1 6 1 +1Score > 24 indicates identity
Score > 19 indicates homology
ATVNTKTGQVVDR + 2 Deamidated (NQ)
4110 347.4511 1385.7753 1385.7514 17.3 1 6 1 +1Score > 21 indicates identity
Score > 19 indicates homology
RLLEAEQISAQK + Deamidated (NQ)
3870 364.5388 1090.5946 1090.6022 -7.03 1 6 0.38 +1Score > 23 indicates identity
Score > 15 indicates homology
KLETPAAFSK
3907 567.2700 1132.5254 1132.5223 2.80 1 6 0.86 +1Score > 23 indicates identity
Score > 18 indicates homology
FSADYQKMK + Oxidation (M)
3945 593.8228 1185.6310 1185.6176 11.4 1 6 0.63 +1Score > 24 indicates identity
Score > 17 indicates homology
IQEAPRDVMK
3979 613.3467 1224.6788 1224.6826 -3.08 0 6 1.1 +1Score > 19 indicates identity LAISHGIAQSTK
3611 416.7450 831.4754 831.4854 -12.0 0 6 0.98 +1Score > 23 indicates identity
Score > 19 indicates homology
FIPNLTK
4085 341.9442 1363.7477 1363.7401 5.58 1 6 0.83 +1Score > 21 indicates identity
Score > 18 indicates homology
QFLFRFEHIK
3724 472.2432 942.4718 942.4771 -5.52 0 6 0.4 +1Score > 21 indicates identity
Score > 15 indicates homology
ASQDPQGIK
3540 377.6817 753.3488 753.3545 -7.47 0 6 1.2 +1Score > 19 indicates identity ETFTQK + Deamidated (NQ)
3877 550.8043 1099.5940 1099.6125 -16.7 0 6 0.69 +1Score > 23 indicates identity
Score > 17 indicates homology
QLEVLEQLK + Deamidated (NQ)
3370 300.6736 599.3326 599.3391 -10.8 0 6 0.56 +1Score > 20 indicates identity
Score > 16 indicates homology
ASLGPR
3843 533.2858 1064.5570 1064.5462 10.2 1 6 0.39 +1Score > 22 indicates identity
Score > 15 indicates homology
SSNLKSSTNK
3707 310.1841 927.5305 927.5277 3.01 0 6 0.87 +1Score > 22 indicates identity
Score > 18 indicates homology
ITEVLPQK + Deamidated (NQ)
3660 443.7654 885.5162 885.5032 14.7 0 6 1 +1Score > 23 indicates identity
Score > 19 indicates homology
ASGITGRPK
4024 641.8340 1281.6534 1281.6717 -14.3 0 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology
DGVIYVALNYR
4082 341.9440 1363.7469 1363.7208 19.1 1 6 0.99 +1Score > 21 indicates identity
Score > 18 indicates homology
EYLLSGGSGARVR
3732 475.2740 948.5334 948.5392 -6.11 1 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology
RSVLYPSK
3742 320.5239 958.5499 958.5447 5.35 0 6 0.59 +1Score > 23 indicates identity
Score > 16 indicates homology
EGLVLSSVR
3818 522.2993 1042.5840 1042.5771 6.68 1 6 0.29 +1Score > 23 indicates identity
Score > 13 indicates homology
QGVEVRLNK + Deamidated (NQ)
3658 443.2476 884.4806 884.4967 -18.2 1 6 0.49 +1Score > 21 indicates identity
Score > 15 indicates homology
KNPQLVGK + 2 Deamidated (NQ)
3514 365.7106 729.4066 729.4173 -14.7 0 6 0.83 +1Score > 24 indicates identity
Score > 18 indicates homology
LGHYLK
3377 306.6968 611.3790 611.3867 -12.5 1 6 0.81 +1Score > 17 indicates identity ARLPR
3786 337.8473 1010.5201 1010.5145 5.52 0 6 0.33 +1Score > 22 indicates identity
Score > 13 indicates homology
TQAIHLDGR + Deamidated (NQ)
3432 653.3291 652.3218 652.3180 5.83 0 6 1.2 +1Score > 19 indicates identity SAQYGK
3418 322.1649 642.3152 642.3085 10.5 0 6 1 +1Score > 18 indicates identity QNPER
3845 534.2622 1066.5098 1066.4931 15.7 0 6 0.35 +1Score > 22 indicates identity
Score > 13 indicates homology
VDGLSEEYR
3604 414.2734 826.5322 826.5276 5.62 0 5 0.43 +1Score > 14 indicates identity AISKPALK
3530 373.7269 745.4392 745.4374 2.47 0 5 0.91 +1Score > 20 indicates identity
Score > 17 indicates homology
LIFQPK + Deamidated (NQ)
4083 341.9440 1363.7469 1363.7493 -1.77 1 5 0.45 +1Score > 21 indicates identity
Score > 14 indicates homology
SLSATISMRTGIK
3579 402.6897 803.3648 803.3524 15.5 0 5 0.8 +1Score > 22 indicates identity
Score > 17 indicates homology
EYAFMK + Oxidation (M)
4126 474.9045 1421.6917 1421.6886 2.18 0 5 1 +1Score > 24 indicates identity
Score > 18 indicates homology
SGNSTTSLPTIDTK + Deamidated (NQ)
3733 317.1887 948.5443 948.5392 5.30 1 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology
LRTNFIGK + Deamidated (NQ)
4263 571.2671 1710.7795 1710.7883 -5.16 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
TTPQGMSPSPPEQAVNA
3846 534.2985 1066.5824 1066.5957 -12.4 0 5 0.36 +1Score > 20 indicates identity
Score > 13 indicates homology
KPGIPTPAMR
3855 360.8557 1079.5453 1079.5546 -8.61 1 5 0.56 +1Score > 24 indicates identity
Score > 15 indicates homology
YRIECAIR
4046 657.8297 1313.6448 1313.6649 -15.3 0 5 1 +1Score > 23 indicates identity
Score > 18 indicates homology
IGPSMTPDELVR
3423 323.2016 644.3886 644.3857 4.56 0 5 1 +1Score > 23 indicates identity
Score > 18 indicates homology
AVNLTK
3856 361.8439 1082.5099 1082.4992 9.85 1 5 0.35 +1Score > 21 indicates identity
Score > 13 indicates homology
SYGSEKANAR + Deamidated (NQ)
4422 889.1217 2664.3433 2664.3846 -15.5 1 5 0.65 +1Score > 23 indicates identity
Score > 16 indicates homology
FLYSAPKPAPEVDLPRNPMHLTR + Oxidation (M)
4099 685.8798 1369.7450 1369.7201 18.2 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
SLTPSGSGGQLPIR + Deamidated (NQ)
4093 456.2549 1365.7429 1365.7365 4.70 0 5 0.52 +1Score > 20 indicates identity
Score > 15 indicates homology
HLQSVIDAQISR
3895 560.8076 1119.6006 1119.5924 7.36 0 5 0.95 +1Score > 23 indicates identity
Score > 17 indicates homology
QQFATAINVK + Deamidated (NQ)
3621 423.2297 844.4448 844.4290 18.7 0 5 1 +1Score > 25 indicates identity
Score > 17 indicates homology
IGEQLQR + 2 Deamidated (NQ)
3702 462.7460 923.4774 923.4600 18.9 1 5 0.78 +1Score > 22 indicates identity
Score > 16 indicates homology
KLQDYEK + Deamidated (NQ)
3881 553.7988 1105.5830 1105.6019 -17.1 0 5 0.7 +1Score > 23 indicates identity
Score > 16 indicates homology
ILYDPATSVK
3472 343.2184 684.4222 684.4283 -8.77 0 5 1.3 +1Score > 18 indicates identity AVGAIVR
3542 380.2002 758.3858 758.3922 -8.43 0 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
KPNGSQK + Deamidated (NQ)
3793 339.5398 1015.5976 1015.5927 4.83 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
KERPALFR
4081 455.2695 1362.7867 1362.7772 6.95 1 5 0.89 +1Score > 17 indicates identity FLRIVFGVQER
4019 640.3124 1278.6102 1278.5993 8.57 0 5 1 +1Score > 24 indicates identity
Score > 17 indicates homology
QADFQTYLHR + Deamidated (NQ)
4322 641.2927 1920.8563 1920.8589 -1.35 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
SDPDDPNTAGKSFLEEAK + Deamidated (NQ)
4236 540.9492 1619.8258 1619.8453 -12.1 1 4 0.47 +1Score > 24 indicates identity
Score > 14 indicates homology
MLLWGRVQSSLASR + Deamidated (NQ); Oxidation (M)
4246 550.6258 1648.8556 1648.8434 7.40 1 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
FTRLTQTANWANAR
4294 603.6430 1807.9072 1807.9389 -17.6 1 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
VMYGAEKIELSELAQK
4290 601.6359 1801.8859 1801.8821 2.10 1 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
QEIYYALAMQRFPR + Deamidated (NQ); Oxidation (M)
3499 360.1794 718.3442 718.3497 -7.61 0 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
LEQSDK
3949 596.8051 1191.5956 1191.6169 -17.8 0 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
SGGAEILGTLMK + Oxidation (M)
3950 597.2871 1192.5596 1192.5403 16.3 0 4 0.65 +1Score > 23 indicates identity
Score > 15 indicates homology
NMPGVLMAMGR + Deamidated (NQ); Oxidation (M)
3704 309.5013 925.4821 925.4981 -17.3 0 4 0.85 +1Score > 21 indicates identity
Score > 16 indicates homology
TAHSLIER
4003 629.3131 1256.6116 1256.6037 6.31 0 4 0.83 +1Score > 23 indicates identity
Score > 16 indicates homology
FTSALSNFDQK
4033 431.2298 1290.6676 1290.6509 12.9 0 4 1 +1Score > 24 indicates identity
Score > 16 indicates homology
LWHFIYAEGR
3985 413.5435 1237.6087 1237.6302 -17.4 0 4 0.82 +1Score > 23 indicates identity
Score > 16 indicates homology
GLATASEHPLDK
3474 344.2071 686.3996 686.4075 -11.4 1 4 0.69 +1Score > 24 indicates identity
Score > 15 indicates homology
VIGRNK + Deamidated (NQ)
3410 318.1992 634.3838 634.3802 5.70 0 4 1.4 +1Score > 18 indicates identity FILSR
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