MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2268 393.2997 392.2924
2269 393.2998 392.2925
2270 393.2998 392.2925
2271 393.2998 392.2925
2272 393.2998 392.2925
2273 393.2998 392.2925
2274 393.2998 392.2925
2275 393.2998 392.2925
2276 393.2998 392.2925
2277 393.2999 392.2926
2278 393.2999 392.2926
2279 393.3000 392.2927
2280 393.3000 392.2927
2281 393.3001 392.2928
2282 395.0763 394.0690
2283 395.0769 394.0696
2284 395.1040 394.0967
2285 395.1336 394.1263
2286 395.1363 394.1290
2287 395.1407 394.1334
2288 395.1695 394.1622
2289 395.1700 394.1627
2290 395.1700 394.1627
2291 395.1700 394.1627
2292 395.1700 394.1627
2293 395.1700 394.1627
2294 395.1701 394.1628
2295 395.1701 394.1628
2296 395.1701 394.1628
2297 395.1701 394.1628
2298 395.1702 394.1629
2299 395.1702 394.1629
2300 395.1703 394.1630
2301 395.1703 394.1630
2302 395.1704 394.1631
2303 395.1704 394.1631
2304 395.1705 394.1632
2305 395.1706 394.1633
2306 395.1707 394.1634
2307 395.1718 394.1645
2308 395.2039 394.1966
2309 395.2060 394.1987
2310 395.2061 394.1988
2311 395.2061 394.1988
2312 395.2063 394.1990
2313 395.2064 394.1991
2314 395.2064 394.1991
2315 395.2065 394.1992
2316 395.2065 394.1992
2317 395.2066 394.1993
2318 395.2070 394.1997
2319 395.2072 394.1999
2320 395.2084 394.2011
2321 395.2248 394.2175
2322 395.2251 394.2178
2323 395.2257 394.2184
2324 395.2603 394.2530
2325 395.2608 394.2535
2326 397.0834 396.0761
2327 397.1183 396.1110
2328 397.1202 396.1129
2329 397.1488 396.1415
2330 397.1491 396.1418
2331 397.1493 396.1420
2332 397.1496 396.1423
2333 397.1498 396.1425
2334 397.1512 396.1439
2335 397.1651 396.1578
2336 397.1851 396.1778
2337 397.1856 396.1783
2338 397.1856 396.1783
2339 397.1857 396.1784
2340 397.1857 396.1784
2341 397.1857 396.1784
2342 397.1858 396.1785
2343 397.1859 396.1786
2344 397.1859 396.1786
2345 397.1859 396.1786
2346 397.1859 396.1786
2347 397.1859 396.1786
2348 397.1859 396.1786
2349 397.1860 396.1787
2350 397.1860 396.1787
2351 397.1860 396.1787
2352 397.1861 396.1788
2353 397.1862 396.1789
2354 397.1866 396.1793
2355 397.1876 396.1803
2356 397.1887 396.1814
2357 397.2043 396.1970
2358 397.2044 396.1971
2359 397.2045 396.1972
2360 397.2046 396.1973
2361 397.2046 396.1973
2362 397.2047 396.1974
2363 397.2049 396.1976
2364 397.2061 396.1988
2365 397.2208 396.2135
2366 397.2216 396.2143
2367 397.2219 396.2146
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