| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 4 |
| MS data file | : | PRT1231_JBEI_4.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 1 May 2025 at 20:40:53 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,452 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 490.5923 | 1468.7551 | 1468.7423 | 8.72 | 1 | 11 | 0.8 | 1Score > 23 indicates identity |
GGAPWIQNDGKVAR + Deamidated (NQ) | |
| 558.3101 | 1114.6056 | 1114.6168 | -10.0 | 1 | 11 | 0.8 | 1Score > 23 indicates identityScore > 23 indicates homology |
KPGSMNLPKK + Oxidation (M) | |
| 392.2203 | 782.4260 | 782.4286 | -3.31 | 0 | 11 | 0.35 | 1Score > 19 indicates identity |
VHSAQIK + Deamidated (NQ) | |
| 458.2818 | 914.5490 | 914.5437 | 5.90 | 1 | 11 | 0.42 | 1Score > 20 indicates identityScore > 20 indicates homology |
AELVKLDK | |
| 345.1781 | 688.3416 | 688.3504 | -12.7 | 1 | 11 | 0.79 | 1Score > 25 indicates identityScore > 22 indicates homology |
QREEK | |
| 417.7201 | 833.4256 | 833.4144 | 13.5 | 0 | 11 | 0.35 | 1Score > 24 indicates identityScore > 19 indicates homology |
AAAGFQNR | |
| 360.2281 | 718.4416 | 718.4411 | 0.76 | 1 | 11 | 0.16 | 1Score > 16 indicates identity |
IMAKLK + Oxidation (M) | |
| 335.1875 | 1002.5407 | 1002.5345 | 6.13 | 1 | 11 | 0.79 | 1Score > 24 indicates identityScore > 22 indicates homology |
LSKEEIER | |
| 576.9819 | 1727.9239 | 1727.9094 | 8.39 | 0 | 11 | 0.14 | 1Score > 22 indicates identityScore > 15 indicates homology |
LLELGVPFESKPEDR | |
| 500.5901 | 1498.7485 | 1498.7515 | -2.01 | 0 | 11 | 0.22 | 1Score > 24 indicates identityScore > 17 indicates homology |
EVLQEESNVQPVK + Deamidated (NQ) | |
| 374.2318 | 746.4490 | 746.4439 | 6.91 | 0 | 11 | 0.3 | 1Score > 18 indicates identity |
ALQLFR | |
| 610.3047 | 1218.5948 | 1218.5914 | 2.82 | 0 | 11 | 0.13 | 1Score > 25 indicates identityScore > 14 indicates homology |
MAIDPQTSSLR + Deamidated (NQ) | |
| 404.2426 | 806.4706 | 806.4650 | 6.95 | 0 | 11 | 0.33 | 1Score > 18 indicates identity |
VVLGYTR | |
| 387.7108 | 773.4070 | 773.4144 | -9.50 | 1 | 11 | 0.93 | 1Score > 26 indicates identityScore > 23 indicates homology |
KGGQTQR | |
| 314.7068 | 627.3990 | 627.3956 | 5.57 | 0 | 10 | 0.33 | 1Score > 18 indicates identity |
TVIPAK | |
| 630.3962 | 629.3889 | 629.3860 | 4.59 | 0 | 10 | 1 | 1Score > 23 indicates identityScore > 23 indicates homology |
AASLIR | |
| 372.2443 | 742.4740 | 742.4701 | 5.33 | 1 | 10 | 0.53 | 1Score > 20 indicates identity |
LQKNLK | |
| 315.7021 | 629.3896 | 629.3860 | 5.72 | 0 | 10 | 0.69 | 1Score > 23 indicates identityScore > 21 indicates homology |
TIAGIR | |
| 585.8220 | 1169.6294 | 1169.6339 | -3.79 | 0 | 10 | 0.76 | 1Score > 22 indicates identity |
CLAVLNARPR + Deamidated (NQ) | |
| 579.3324 | 1156.6502 | 1156.6452 | 4.40 | 1 | 10 | 0.5 | 1Score > 23 indicates identityScore > 20 indicates homology |
KIISELDPSR | |
| 374.1962 | 746.3778 | 746.3745 | 4.49 | 0 | 10 | 0.59 | 1Score > 26 indicates identityScore > 20 indicates homology |
VICEAR | |
| 567.2785 | 1132.5424 | 1132.5546 | -10.8 | 1 | 10 | 0.21 | 1Score > 24 indicates identityScore > 16 indicates homology |
DMSEGIRTPK | |
| 346.7118 | 691.4090 | 691.4017 | 10.6 | 1 | 10 | 0.49 | 1Score > 20 indicates identity |
QRLFK + Deamidated (NQ) | |
| 525.5788 | 1573.7146 | 1573.7009 | 8.72 | 0 | 10 | 0.67 | 1Score > 22 indicates identityScore > 21 indicates homology |
VQNGAVTWESDPNR + 2 Deamidated (NQ) | |
| 308.8326 | 923.4760 | 923.4600 | 17.3 | 1 | 10 | 0.64 | 1Score > 22 indicates identityScore > 21 indicates homology |
KLQDYEK + Deamidated (NQ) | |
| 402.7295 | 803.4444 | 803.4323 | 15.1 | 0 | 10 | 0.84 | 1Score > 24 indicates identityScore > 22 indicates homology |
LQLMQR + Oxidation (M) | |
| 435.7583 | 869.5020 | 869.4872 | 17.1 | 0 | 10 | 0.48 | 1Score > 19 indicates identity |
AGAIFHVR | |
| 461.2585 | 920.5024 | 920.4868 | 17.0 | 0 | 10 | 0.9 | 1Score > 22 indicates identityScore > 22 indicates homology |
IGFAWATR | |
| 768.8905 | 1535.7664 | 1535.7654 | 0.70 | 1 | 10 | 0.25 | 1Score > 24 indicates identityScore > 16 indicates homology |
SEMWEVTNKAVVK + Oxidation (M) | |
| 392.7452 | 783.4758 | 783.4827 | -8.78 | 1 | 10 | 0.44 | 1Score > 19 indicates identity |
RRPSLR | |
| 628.4062 | 627.3989 | 627.3956 | 5.37 | 0 | 10 | 0.38 | 1Score > 18 indicates identity |
TVIPAK | |
| 446.7631 | 891.5116 | 891.5066 | 5.71 | 0 | 10 | 0.51 | 1Score > 19 indicates identity |
LVSQGLFK + Deamidated (NQ) | |
| 405.2256 | 808.4366 | 808.4330 | 4.46 | 0 | 10 | 0.51 | 1Score > 20 indicates identityScore > 19 indicates homology |
VYALSEK | |
| 564.8073 | 1127.6000 | 1127.6186 | -16.5 | 0 | 10 | 0.18 | 1Score > 22 indicates identityScore > 15 indicates homology |
IDILINNVGR + 2 Deamidated (NQ) | |
| 413.2176 | 1648.8413 | 1648.8169 | 14.8 | 1 | 10 | 0.29 | 1Score > 24 indicates identityScore > 17 indicates homology |
TNAEVDTRYPNLTR | |
| 662.7964 | 1323.5782 | 1323.5877 | -7.16 | 1 | 10 | 0.14 | 1Score > 20 indicates identityScore > 14 indicates homology |
VRMYDAPQNGR + 2 Deamidated (NQ); Oxidation (M) | |
| 310.5119 | 928.5139 | 928.5090 | 5.26 | 1 | 10 | 0.97 | 1Score > 24 indicates identityScore > 22 indicates homology |
IRENLER | |
| 508.2828 | 1014.5510 | 1014.5458 | 5.18 | 1 | 10 | 0.98 | 1Score > 24 indicates identityScore > 22 indicates homology |
QLQDVQRK + Deamidated (NQ) | |
| 397.2159 | 792.4172 | 792.4164 | 1.11 | 0 | 10 | 0.16 | 1Score > 24 indicates identityScore > 14 indicates homology |
SLGMTLR + Oxidation (M) | |
| 360.7259 | 719.4372 | 719.4330 | 5.91 | 0 | 10 | 0.23 | 1Score > 16 indicates identity |
YLVAVR | |
| 368.1911 | 734.3676 | 734.3745 | -9.30 | 1 | 10 | 0.72 | 1Score > 23 indicates identityScore > 21 indicates homology |
LGRMNK + Deamidated (NQ); Oxidation (M) | |
| 607.8169 | 1213.6192 | 1213.6237 | -3.68 | 1 | 10 | 1 | 1Score > 23 indicates identityScore > 22 indicates homology |
NCLQKIAPDR | |
| 402.6894 | 803.3642 | 803.3596 | 5.82 | 0 | 9 | 0.9 | 1Score > 22 indicates identityScore > 21 indicates homology |
QSAHSMK + Oxidation (M) | |
| 544.7922 | 1087.5698 | 1087.5509 | 17.4 | 0 | 9 | 0.43 | 1Score > 25 indicates identityScore > 18 indicates homology |
NDLELNTLR + Deamidated (NQ) | |
| 324.1751 | 646.3356 | 646.3286 | 10.9 | 1 | 9 | 0.77 | 1Score > 26 indicates identityScore > 21 indicates homology |
QEKDK | |
| 393.2499 | 784.4852 | 784.4807 | 5.82 | 0 | 9 | 0.7 | 1Score > 20 indicates identityScore > 20 indicates homology |
LAQVVQK | |
| 401.8653 | 1202.5741 | 1202.5527 | 17.8 | 0 | 9 | 0.54 | 1Score > 24 indicates identityScore > 19 indicates homology |
QSIEAAEGNQR + Deamidated (NQ) | |
| 304.8359 | 911.4859 | 911.4825 | 3.75 | 1 | 9 | 0.3 | 1Score > 20 indicates identityScore > 17 indicates homology |
TKEHIER | |
| 534.2730 | 533.2657 | 533.2558 | 18.7 | 0 | 9 | 1 | 1Score > 23 indicates identityScore > 22 indicates homology |
ASSNR | |
| 458.2696 | 914.5246 | 914.5185 | 6.73 | 1 | 9 | 0.41 | 1Score > 23 indicates identityScore > 18 indicates homology |
KAGLEELR | |
| 318.1808 | 634.3470 | 634.3438 | 5.05 | 0 | 9 | 0.81 | 1Score > 22 indicates identityScore > 21 indicates homology |
YLSPR | |
| 332.1809 | 993.5209 | 993.5131 | 7.83 | 0 | 9 | 0.93 | 1Score > 22 indicates identityScore > 21 indicates homology |
FTTALQANK + Deamidated (NQ) | |
| 373.2130 | 1116.6172 | 1116.5961 | 18.9 | 1 | 9 | 0.63 | 1Score > 23 indicates identityScore > 19 indicates homology |
QREALQMLK + Deamidated (NQ) | |
| 441.8929 | 1322.6569 | 1322.6652 | -6.32 | 1 | 9 | 0.66 | 1Score > 24 indicates identityScore > 20 indicates homology |
IKFEMEQNLR + Oxidation (M) | |
| 515.7744 | 1029.5342 | 1029.5243 | 9.64 | 0 | 9 | 0.57 | 1Score > 24 indicates identityScore > 19 indicates homology |
LNGYPVPNR + Deamidated (NQ) | |
| 394.7186 | 787.4226 | 787.4188 | 4.92 | 0 | 9 | 1 | 1Score > 26 indicates identityScore > 21 indicates homology |
LSAAAEAR | |
| 541.3486 | 540.3413 | 540.3384 | 5.45 | 0 | 9 | 0.71 | 1Score > 20 indicates identity |
GVIPR | |
| 401.5375 | 1201.5907 | 1201.6138 | -19.3 | 1 | 9 | 0.26 | 1Score > 24 indicates identityScore > 16 indicates homology |
GCPKFGRPQR | |
| 409.2265 | 816.4384 | 816.4341 | 5.33 | 0 | 9 | 0.87 | 1Score > 25 indicates identityScore > 21 indicates homology |
GLASLNNK + Deamidated (NQ) | |
| 387.8862 | 1160.6368 | 1160.6315 | 4.50 | 1 | 9 | 0.69 | 1Score > 23 indicates identityScore > 20 indicates homology |
RHTWGHLVR | |
| 338.1838 | 674.3530 | 674.3599 | -10.2 | 0 | 9 | 0.23 | 1Score > 25 indicates identityScore > 15 indicates homology |
QTAEVK | |
| 450.7817 | 899.5488 | 899.5440 | 5.40 | 0 | 9 | 0.4 | 1Score > 20 indicates identityScore > 17 indicates homology |
SLLGLLER | |
| 360.1852 | 718.3558 | 718.3497 | 8.54 | 0 | 9 | 1 | 1Score > 25 indicates identityScore > 21 indicates homology |
LGSDAEK | |
| 560.8251 | 1119.6356 | 1119.6136 | 19.7 | 1 | 9 | 0.3 | 1Score > 20 indicates identityScore > 16 indicates homology |
IKTSDGSVSVK | |
| 401.7449 | 801.4752 | 801.4708 | 5.50 | 0 | 9 | 0.98 | 1Score > 23 indicates identityScore > 21 indicates homology |
VLNISTR | |
| 705.3403 | 1408.6660 | 1408.6657 | 0.27 | 1 | 9 | 0.72 | 1Score > 23 indicates identityScore > 20 indicates homology |
ITGGNDKQGFPMK + Deamidated (NQ); Oxidation (M) | |
| 563.7850 | 1125.5554 | 1125.5600 | -4.08 | 1 | 9 | 0.37 | 1Score > 23 indicates identityScore > 17 indicates homology |
MQSINARYK + Oxidation (M) | |
| 386.5577 | 1156.6513 | 1156.6312 | 17.3 | 1 | 9 | 0.26 | 1Score > 23 indicates identityScore > 15 indicates homology |
RALENSALQR | |
| 475.2180 | 948.4214 | 948.4148 | 6.97 | 0 | 9 | 0.2 | 1Score > 22 indicates identityScore > 14 indicates homology |
TGNEASVDR + Deamidated (NQ) | |
| 610.8328 | 1219.6510 | 1219.6309 | 16.5 | 1 | 9 | 0.38 | 1Score > 23 indicates identityScore > 17 indicates homology |
NGGSITPRYQK | |
| 531.7852 | 1061.5558 | 1061.5757 | -18.7 | 0 | 9 | 0.62 | 1Score > 24 indicates identityScore > 19 indicates homology |
VDFLNLAAAK + Deamidated (NQ) | |
| 640.8315 | 1279.6484 | 1279.6673 | -14.7 | 1 | 8 | 1 | 1Score > 23 indicates identityScore > 21 indicates homology |
SNRVFYPLQR + Deamidated (NQ) | |
| 503.2988 | 1004.5830 | 1004.5920 | -8.88 | 1 | 8 | 0.68 | 1Score > 19 indicates identity |
PFLARVFR | |
| 372.1959 | 1113.5659 | 1113.5488 | 15.3 | 0 | 8 | 0.72 | 1Score > 22 indicates identityScore > 20 indicates homology |
MVVNQQHLK + 2 Deamidated (NQ); Oxidation (M) | |
| 478.2643 | 954.5140 | 954.5148 | -0.74 | 1 | 8 | 0.22 | 1Score > 21 indicates identityScore > 14 indicates homology |
NLYRHPR | |
| 569.3691 | 568.3618 | 568.3697 | -13.8 | 0 | 8 | 0.24 | 1Score > 15 indicates identity |
IPIAR | |
| 658.8187 | 1315.6228 | 1315.6255 | -2.04 | 0 | 8 | 0.42 | 1Score > 23 indicates identityScore > 17 indicates homology |
VNIELAQDQER + 2 Deamidated (NQ) | |
| 508.8070 | 1015.5994 | 1015.5848 | 14.4 | 1 | 8 | 0.8 | 1Score > 20 indicates identity |
RALTPTMVK | |
| 478.2640 | 954.5134 | 954.5208 | -7.72 | 1 | 8 | 0.75 | 1Score > 21 indicates identityScore > 19 indicates homology |
ASLMKVYK + Oxidation (M) | |
| 314.6758 | 627.3370 | 627.3340 | 4.86 | 0 | 8 | 0.77 | 1Score > 20 indicates identity |
IENPR | |
| 330.6703 | 659.3260 | 659.3238 | 3.35 | 0 | 8 | 0.96 | 1Score > 26 indicates identityScore > 20 indicates homology |
GADLER | |
| 314.6996 | 627.3846 | 627.3816 | 4.80 | 0 | 8 | 0.42 | 1Score > 17 indicates identity |
RPTVR | |
| 400.2140 | 1197.6202 | 1197.5990 | 17.7 | 0 | 8 | 0.45 | 1Score > 22 indicates identityScore > 17 indicates homology |
SSPISSSSPPPR | |
| 319.1779 | 954.5119 | 954.5148 | -3.02 | 1 | 8 | 0.6 | 1Score > 21 indicates identityScore > 18 indicates homology |
NLYRHPR | |
| 506.7689 | 1011.5232 | 1011.5171 | 6.04 | 1 | 8 | 0.28 | 1Score > 20 indicates identityScore > 15 indicates homology |
KYGGLMTAR + Oxidation (M) | |
| 567.3039 | 1132.5932 | 1132.5877 | 4.92 | 0 | 8 | 0.22 | 1Score > 23 indicates identityScore > 14 indicates homology |
GALGDGVEFLR | |
| 372.5421 | 1114.6045 | 1114.6234 | -17.0 | 0 | 8 | 0.8 | 1Score > 23 indicates identityScore > 19 indicates homology |
QGTLLDELVK | |
| 341.6995 | 681.3844 | 681.3810 | 5.11 | 0 | 8 | 0.82 | 1Score > 20 indicates identity |
DIIGHK | |
| 363.1985 | 1086.5737 | 1086.5557 | 16.6 | 0 | 8 | 0.88 | 1Score > 24 indicates identityScore > 20 indicates homology |
AQTAPSAEIAK + Deamidated (NQ) | |
| 449.7921 | 897.5696 | 897.5760 | -7.03 | 1 | 8 | 0.33 | 1Score > 15 indicates identity |
IIIIDRR | |
| 322.7038 | 643.3930 | 643.3905 | 4.00 | 0 | 8 | 0.49 | 1Score > 25 indicates identityScore > 17 indicates homology |
LGDLVK | |
| 361.7150 | 721.4154 | 721.4123 | 4.40 | 0 | 8 | 0.27 | 1Score > 20 indicates identityScore > 15 indicates homology |
YVVSVR | |
| 523.7592 | 1045.5038 | 1045.5226 | -17.9 | 0 | 8 | 0.73 | 1Score > 24 indicates identityScore > 19 indicates homology |
NADNIIVMR + Deamidated (NQ) | |
| 323.1911 | 644.3676 | 644.3605 | 11.0 | 1 | 8 | 0.57 | 1Score > 24 indicates identityScore > 18 indicates homology |
NDRIK | |
| 749.0688 | 2244.1846 | 2244.1559 | 12.8 | 0 | 8 | 1 | 1Score > 21 indicates identityScore > 20 indicates homology |
MGLGVLEDTALAQVPGTSDILK + Deamidated (NQ); Oxidation (M) | |
| 351.2215 | 700.4284 | 700.4232 | 7.55 | 0 | 8 | 0.7 | 1Score > 22 indicates identityScore > 19 indicates homology |
LTQAIR | |
| 451.7715 | 901.5284 | 901.5120 | 18.2 | 0 | 8 | 1 | 1Score > 21 indicates identityScore > 20 indicates homology |
SLDLDIVK | |
| 347.6908 | 693.3670 | 693.3632 | 5.52 | 0 | 8 | 1 | 1Score > 21 indicates identityScore > 20 indicates homology |
ICFVR | |
| 581.3259 | 1160.6372 | 1160.6414 | -3.61 | 1 | 8 | 0.78 | 1Score > 23 indicates identityScore > 19 indicates homology |
TREHAVPVPR | |
| 632.3646 | 631.3573 | 631.3653 | -12.7 | 1 | 8 | 0.93 | 1Score > 26 indicates identityScore > 20 indicates homology |
VRETK |
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