MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 4247)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4154 490.5923 1468.7551 1468.7423 8.72 1 11 0.8 +1Score > 23 indicates identity GGAPWIQNDGKVAR + Deamidated (NQ)
3888 558.3101 1114.6056 1114.6168 -10.0 1 11 0.8 +1Score > 23 indicates identity
Score > 23 indicates homology
KPGSMNLPKK + Oxidation (M)
3554 392.2203 782.4260 782.4286 -3.31 0 11 0.35 +1Score > 19 indicates identity VHSAQIK + Deamidated (NQ)
3694 458.2818 914.5490 914.5437 5.90 1 11 0.42 +1Score > 20 indicates identity
Score > 20 indicates homology
AELVKLDK
3477 345.1781 688.3416 688.3504 -12.7 1 11 0.79 +1Score > 25 indicates identity
Score > 22 indicates homology
QREEK
3612 417.7201 833.4256 833.4144 13.5 0 11 0.35 +1Score > 24 indicates identity
Score > 19 indicates homology
AAAGFQNR
3501 360.2281 718.4416 718.4411 0.76 1 11 0.16 +1Score > 16 indicates identity IMAKLK + Oxidation (M)
3781 335.1875 1002.5407 1002.5345 6.13 1 11 0.79 +1Score > 24 indicates identity
Score > 22 indicates homology
LSKEEIER
4273 576.9819 1727.9239 1727.9094 8.39 0 11 0.14 +1Score > 22 indicates identity
Score > 15 indicates homology
LLELGVPFESKPEDR
4168 500.5901 1498.7485 1498.7515 -2.01 0 11 0.22 +1Score > 24 indicates identity
Score > 17 indicates homology
EVLQEESNVQPVK + Deamidated (NQ)
3534 374.2318 746.4490 746.4439 6.91 0 11 0.3 +1Score > 18 indicates identity ALQLFR
3974 610.3047 1218.5948 1218.5914 2.82 0 11 0.13 +1Score > 25 indicates identity
Score > 14 indicates homology
MAIDPQTSSLR + Deamidated (NQ)
3585 404.2426 806.4706 806.4650 6.95 0 11 0.33 +1Score > 18 indicates identity VVLGYTR
3549 387.7108 773.4070 773.4144 -9.50 1 11 0.93 +1Score > 26 indicates identity
Score > 23 indicates homology
KGGQTQR
3397 314.7068 627.3990 627.3956 5.57 0 10 0.33 +1Score > 18 indicates identity TVIPAK
3403 630.3962 629.3889 629.3860 4.59 0 10 1 +1Score > 23 indicates identity
Score > 23 indicates homology
AASLIR
3524 372.2443 742.4740 742.4701 5.33 1 10 0.53 +1Score > 20 indicates identity LQKNLK
3404 315.7021 629.3896 629.3860 5.72 0 10 0.69 +1Score > 23 indicates identity
Score > 21 indicates homology
TIAGIR
3936 585.8220 1169.6294 1169.6339 -3.79 0 10 0.76 +1Score > 22 indicates identity CLAVLNARPR + Deamidated (NQ)
3926 579.3324 1156.6502 1156.6452 4.40 1 10 0.5 +1Score > 23 indicates identity
Score > 20 indicates homology
KIISELDPSR
3531 374.1962 746.3778 746.3745 4.49 0 10 0.59 +1Score > 26 indicates identity
Score > 20 indicates homology
VICEAR
3908 567.2785 1132.5424 1132.5546 -10.8 1 10 0.21 +1Score > 24 indicates identity
Score > 16 indicates homology
DMSEGIRTPK
3480 346.7118 691.4090 691.4017 10.6 1 10 0.49 +1Score > 20 indicates identity QRLFK + Deamidated (NQ)
4204 525.5788 1573.7146 1573.7009 8.72 0 10 0.67 +1Score > 22 indicates identity
Score > 21 indicates homology
VQNGAVTWESDPNR + 2 Deamidated (NQ)
3701 308.8326 923.4760 923.4600 17.3 1 10 0.64 +1Score > 22 indicates identity
Score > 21 indicates homology
KLQDYEK + Deamidated (NQ)
3581 402.7295 803.4444 803.4323 15.1 0 10 0.84 +1Score > 24 indicates identity
Score > 22 indicates homology
LQLMQR + Oxidation (M)
3641 435.7583 869.5020 869.4872 17.1 0 10 0.48 +1Score > 19 indicates identity AGAIFHVR
3700 461.2585 920.5024 920.4868 17.0 0 10 0.9 +1Score > 22 indicates identity
Score > 22 indicates homology
IGFAWATR
4186 768.8905 1535.7664 1535.7654 0.70 1 10 0.25 +1Score > 24 indicates identity
Score > 16 indicates homology
SEMWEVTNKAVVK + Oxidation (M)
3555 392.7452 783.4758 783.4827 -8.78 1 10 0.44 +1Score > 19 indicates identity RRPSLR
3396 628.4062 627.3989 627.3956 5.37 0 10 0.38 +1Score > 18 indicates identity TVIPAK
3671 446.7631 891.5116 891.5066 5.71 0 10 0.51 +1Score > 19 indicates identity LVSQGLFK + Deamidated (NQ)
3586 405.2256 808.4366 808.4330 4.46 0 10 0.51 +1Score > 20 indicates identity
Score > 19 indicates homology
VYALSEK
3901 564.8073 1127.6000 1127.6186 -16.5 0 10 0.18 +1Score > 22 indicates identity
Score > 15 indicates homology
IDILINNVGR + 2 Deamidated (NQ)
4245 413.2176 1648.8413 1648.8169 14.8 1 10 0.29 +1Score > 24 indicates identity
Score > 17 indicates homology
TNAEVDTRYPNLTR
4059 662.7964 1323.5782 1323.5877 -7.16 1 10 0.14 +1Score > 20 indicates identity
Score > 14 indicates homology
VRMYDAPQNGR + 2 Deamidated (NQ); Oxidation (M)
3709 310.5119 928.5139 928.5090 5.26 1 10 0.97 +1Score > 24 indicates identity
Score > 22 indicates homology
IRENLER
3792 508.2828 1014.5510 1014.5458 5.18 1 10 0.98 +1Score > 24 indicates identity
Score > 22 indicates homology
QLQDVQRK + Deamidated (NQ)
3570 397.2159 792.4172 792.4164 1.11 0 10 0.16 +1Score > 24 indicates identity
Score > 14 indicates homology
SLGMTLR + Oxidation (M)
3504 360.7259 719.4372 719.4330 5.91 0 10 0.23 +1Score > 16 indicates identity YLVAVR
3516 368.1911 734.3676 734.3745 -9.30 1 10 0.72 +1Score > 23 indicates identity
Score > 21 indicates homology
LGRMNK + Deamidated (NQ); Oxidation (M)
3969 607.8169 1213.6192 1213.6237 -3.68 1 10 1 +1Score > 23 indicates identity
Score > 22 indicates homology
NCLQKIAPDR
3578 402.6894 803.3642 803.3596 5.82 0 9 0.9 +1Score > 22 indicates identity
Score > 21 indicates homology
QSAHSMK + Oxidation (M)
3867 544.7922 1087.5698 1087.5509 17.4 0 9 0.43 +1Score > 25 indicates identity
Score > 18 indicates homology
NDLELNTLR + Deamidated (NQ)
3426 324.1751 646.3356 646.3286 10.9 1 9 0.77 +1Score > 26 indicates identity
Score > 21 indicates homology
QEKDK
3560 393.2499 784.4852 784.4807 5.82 0 9 0.7 +1Score > 20 indicates identity
Score > 20 indicates homology
LAQVVQK
3961 401.8653 1202.5741 1202.5527 17.8 0 9 0.54 +1Score > 24 indicates identity
Score > 19 indicates homology
QSIEAAEGNQR + Deamidated (NQ)
3687 304.8359 911.4859 911.4825 3.75 1 9 0.3 +1Score > 20 indicates identity
Score > 17 indicates homology
TKEHIER
3343 534.2730 533.2657 533.2558 18.7 0 9 1 +1Score > 23 indicates identity
Score > 22 indicates homology
ASSNR
3693 458.2696 914.5246 914.5185 6.73 1 9 0.41 +1Score > 23 indicates identity
Score > 18 indicates homology
KAGLEELR
3409 318.1808 634.3470 634.3438 5.05 0 9 0.81 +1Score > 22 indicates identity
Score > 21 indicates homology
YLSPR
3770 332.1809 993.5209 993.5131 7.83 0 9 0.93 +1Score > 22 indicates identity
Score > 21 indicates homology
FTTALQANK + Deamidated (NQ)
3891 373.2130 1116.6172 1116.5961 18.9 1 9 0.63 +1Score > 23 indicates identity
Score > 19 indicates homology
QREALQMLK + Deamidated (NQ)
4056 441.8929 1322.6569 1322.6652 -6.32 1 9 0.66 +1Score > 24 indicates identity
Score > 20 indicates homology
IKFEMEQNLR + Oxidation (M)
3811 515.7744 1029.5342 1029.5243 9.64 0 9 0.57 +1Score > 24 indicates identity
Score > 19 indicates homology
LNGYPVPNR + Deamidated (NQ)
3563 394.7186 787.4226 787.4188 4.92 0 9 1 +1Score > 26 indicates identity
Score > 21 indicates homology
LSAAAEAR
3345 541.3486 540.3413 540.3384 5.45 0 9 0.71 +1Score > 20 indicates identity GVIPR
3960 401.5375 1201.5907 1201.6138 -19.3 1 9 0.26 +1Score > 24 indicates identity
Score > 16 indicates homology
GCPKFGRPQR
3591 409.2265 816.4384 816.4341 5.33 0 9 0.87 +1Score > 25 indicates identity
Score > 21 indicates homology
GLASLNNK + Deamidated (NQ)
3932 387.8862 1160.6368 1160.6315 4.50 1 9 0.69 +1Score > 23 indicates identity
Score > 20 indicates homology
RHTWGHLVR
3463 338.1838 674.3530 674.3599 -10.2 0 9 0.23 +1Score > 25 indicates identity
Score > 15 indicates homology
QTAEVK
3677 450.7817 899.5488 899.5440 5.40 0 9 0.4 +1Score > 20 indicates identity
Score > 17 indicates homology
SLLGLLER
3500 360.1852 718.3558 718.3497 8.54 0 9 1 +1Score > 25 indicates identity
Score > 21 indicates homology
LGSDAEK
3896 560.8251 1119.6356 1119.6136 19.7 1 9 0.3 +1Score > 20 indicates identity
Score > 16 indicates homology
IKTSDGSVSVK
3577 401.7449 801.4752 801.4708 5.50 0 9 0.98 +1Score > 23 indicates identity
Score > 21 indicates homology
VLNISTR
4120 705.3403 1408.6660 1408.6657 0.27 1 9 0.72 +1Score > 23 indicates identity
Score > 20 indicates homology
ITGGNDKQGFPMK + Deamidated (NQ); Oxidation (M)
3900 563.7850 1125.5554 1125.5600 -4.08 1 9 0.37 +1Score > 23 indicates identity
Score > 17 indicates homology
MQSINARYK + Oxidation (M)
3927 386.5577 1156.6513 1156.6312 17.3 1 9 0.26 +1Score > 23 indicates identity
Score > 15 indicates homology
RALENSALQR
3730 475.2180 948.4214 948.4148 6.97 0 9 0.2 +1Score > 22 indicates identity
Score > 14 indicates homology
TGNEASVDR + Deamidated (NQ)
3975 610.8328 1219.6510 1219.6309 16.5 1 9 0.38 +1Score > 23 indicates identity
Score > 17 indicates homology
NGGSITPRYQK
3841 531.7852 1061.5558 1061.5757 -18.7 0 9 0.62 +1Score > 24 indicates identity
Score > 19 indicates homology
VDFLNLAAAK + Deamidated (NQ)
4020 640.8315 1279.6484 1279.6673 -14.7 1 8 1 +1Score > 23 indicates identity
Score > 21 indicates homology
SNRVFYPLQR + Deamidated (NQ)
3783 503.2988 1004.5830 1004.5920 -8.88 1 8 0.68 +1Score > 19 indicates identity PFLARVFR
3885 372.1959 1113.5659 1113.5488 15.3 0 8 0.72 +1Score > 22 indicates identity
Score > 20 indicates homology
MVVNQQHLK + 2 Deamidated (NQ); Oxidation (M)
3741 478.2643 954.5140 954.5148 -0.74 1 8 0.22 +1Score > 21 indicates identity
Score > 14 indicates homology
NLYRHPR
3361 569.3691 568.3618 568.3697 -13.8 0 8 0.24 +1Score > 15 indicates identity IPIAR
4047 658.8187 1315.6228 1315.6255 -2.04 0 8 0.42 +1Score > 23 indicates identity
Score > 17 indicates homology
VNIELAQDQER + 2 Deamidated (NQ)
3794 508.8070 1015.5994 1015.5848 14.4 1 8 0.8 +1Score > 20 indicates identity RALTPTMVK
3739 478.2640 954.5134 954.5208 -7.72 1 8 0.75 +1Score > 21 indicates identity
Score > 19 indicates homology
ASLMKVYK + Oxidation (M)
3394 314.6758 627.3370 627.3340 4.86 0 8 0.77 +1Score > 20 indicates identity IENPR
3445 330.6703 659.3260 659.3238 3.35 0 8 0.96 +1Score > 26 indicates identity
Score > 20 indicates homology
GADLER
3395 314.6996 627.3846 627.3816 4.80 0 8 0.42 +1Score > 17 indicates identity RPTVR
3954 400.2140 1197.6202 1197.5990 17.7 0 8 0.45 +1Score > 22 indicates identity
Score > 17 indicates homology
SSPISSSSPPPR
3738 319.1779 954.5119 954.5148 -3.02 1 8 0.6 +1Score > 21 indicates identity
Score > 18 indicates homology
NLYRHPR
3789 506.7689 1011.5232 1011.5171 6.04 1 8 0.28 +1Score > 20 indicates identity
Score > 15 indicates homology
KYGGLMTAR + Oxidation (M)
3909 567.3039 1132.5932 1132.5877 4.92 0 8 0.22 +1Score > 23 indicates identity
Score > 14 indicates homology
GALGDGVEFLR
3887 372.5421 1114.6045 1114.6234 -17.0 0 8 0.8 +1Score > 23 indicates identity
Score > 19 indicates homology
QGTLLDELVK
3469 341.6995 681.3844 681.3810 5.11 0 8 0.82 +1Score > 20 indicates identity DIIGHK
3865 363.1985 1086.5737 1086.5557 16.6 0 8 0.88 +1Score > 24 indicates identity
Score > 20 indicates homology
AQTAPSAEIAK + Deamidated (NQ)
3673 449.7921 897.5696 897.5760 -7.03 1 8 0.33 +1Score > 15 indicates identity IIIIDRR
3420 322.7038 643.3930 643.3905 4.00 0 8 0.49 +1Score > 25 indicates identity
Score > 17 indicates homology
LGDLVK
3506 361.7150 721.4154 721.4123 4.40 0 8 0.27 +1Score > 20 indicates identity
Score > 15 indicates homology
YVVSVR
3827 523.7592 1045.5038 1045.5226 -17.9 0 8 0.73 +1Score > 24 indicates identity
Score > 19 indicates homology
NADNIIVMR + Deamidated (NQ)
3422 323.1911 644.3676 644.3605 11.0 1 8 0.57 +1Score > 24 indicates identity
Score > 18 indicates homology
NDRIK
4376 749.0688 2244.1846 2244.1559 12.8 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology
MGLGVLEDTALAQVPGTSDILK + Deamidated (NQ); Oxidation (M)
3488 351.2215 700.4284 700.4232 7.55 0 8 0.7 +1Score > 22 indicates identity
Score > 19 indicates homology
LTQAIR
3681 451.7715 901.5284 901.5120 18.2 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology
SLDLDIVK
3481 347.6908 693.3670 693.3632 5.52 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology
ICFVR
3933 581.3259 1160.6372 1160.6414 -3.61 1 8 0.78 +1Score > 23 indicates identity
Score > 19 indicates homology
TREHAVPVPR
3406 632.3646 631.3573 631.3653 -12.7 1 8 0.93 +1Score > 26 indicates identity
Score > 20 indicates homology
VRETK
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