MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
768 327.1797 326.1724
769 327.1797 326.1724
770 327.1798 326.1725
771 327.1798 326.1725
772 327.1798 326.1725
773 327.1799 326.1726
774 327.1799 326.1726
775 327.1799 326.1726
776 327.1800 326.1727
777 327.1997 326.1924
778 327.1998 326.1925
779 327.2000 326.1927
780 327.2000 326.1927
781 327.2002 326.1929
782 327.2031 326.1958
783 327.2164 326.2091
784 327.2173 326.2100
785 328.0168 327.0095
786 328.0618 327.0545
787 328.2131 327.2058
788 329.0210 328.0137
789 329.0556 328.0483
790 329.1250 328.1177
791 329.1264 328.1191
792 329.1375 328.1302
793 329.1378 328.1305
794 329.1379 328.1306
795 329.1587 328.1514
796 329.1588 328.1515
797 329.1589 328.1516
798 329.1589 328.1516
799 329.1589 328.1516
800 329.1589 328.1516
801 329.1589 328.1516
802 329.1590 328.1517
803 329.1590 328.1517
804 329.1591 328.1518
805 329.1593 328.1520
806 329.1598 328.1525
807 329.1759 328.1686
808 329.1767 328.1694
809 329.1767 328.1694
810 329.2159 328.2086
811 330.0412 329.0339
812 330.0776 329.0703
813 330.2290 329.2217
814 330.2291 329.2218
815 331.0714 330.0641
816 331.0795 330.0722
817 331.1167 330.1094
818 331.1169 330.1096
819 331.1169 330.1096
820 331.1169 330.1096
821 331.1451 330.1378
822 331.1532 330.1459
823 331.1532 330.1459
824 331.1532 330.1459
825 331.1533 330.1460
826 331.1533 330.1460
827 331.1533 330.1460
828 331.1533 330.1460
829 331.1534 330.1461
830 331.1534 330.1461
831 331.1534 330.1461
832 331.1536 330.1463
833 331.1537 330.1464
834 331.1539 330.1466
835 331.1540 330.1467
836 331.1768 330.1695
837 331.1768 330.1695
838 331.1891 330.1818
839 331.1892 330.1819
840 331.1893 330.1820
841 331.1893 330.1820
842 331.1895 330.1822
843 331.1895 330.1822
844 331.1898 330.1825
845 331.1900 330.1827
846 331.2104 330.2031
847 331.2109 330.2036
848 332.0312 331.0239
849 332.2085 331.2012
850 332.2085 331.2012
851 332.2086 331.2013
852 333.0558 332.0485
853 333.0566 332.0493
854 333.0569 332.0496
855 333.0948 332.0875
856 333.0956 332.0883
857 333.0957 332.0884
858 333.1319 332.1246
859 333.1321 332.1248
860 333.1323 332.1250
861 333.1324 332.1251
862 333.1324 332.1251
863 333.1325 332.1252
864 333.1326 332.1253
865 333.1326 332.1253
866 333.1326 332.1253
867 333.1326 332.1253
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