MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
468 318.1928 317.1855
469 319.0795 318.0722
470 319.1140 318.1067
471 319.1165 318.1092
472 319.1165 318.1092
473 319.1166 318.1093
474 319.1167 318.1094
475 319.1167 318.1094
476 319.1167 318.1094
477 319.1167 318.1094
478 319.1168 318.1095
479 319.1168 318.1095
480 319.1168 318.1095
481 319.1168 318.1095
482 319.1168 318.1095
483 319.1169 318.1096
484 319.1169 318.1096
485 319.1184 318.1111
486 319.1530 318.1457
487 319.1530 318.1457
488 319.1530 318.1457
489 319.1531 318.1458
490 319.1531 318.1458
491 319.1532 318.1459
492 319.1532 318.1459
493 319.1532 318.1459
494 319.1532 318.1459
495 319.1533 318.1460
496 319.1533 318.1460
497 319.1533 318.1460
498 319.1533 318.1460
499 319.1534 318.1461
500 319.1534 318.1461
501 319.1534 318.1461
502 319.1535 318.1462
503 319.1535 318.1462
504 319.1535 318.1462
505 319.1536 318.1463
506 319.1536 318.1463
507 319.1537 318.1464
508 319.1537 318.1464
509 319.1667 318.1594
510 319.1667 318.1594
511 319.1892 318.1819
512 319.1894 318.1821
513 319.1894 318.1821
514 319.1895 318.1822
515 319.1895 318.1822
516 319.1896 318.1823
517 319.1896 318.1823
518 319.1896 318.1823
519 319.1897 318.1824
520 319.1898 318.1825
521 319.1898 318.1825
522 319.1899 318.1826
523 319.2258 318.2185
524 319.2260 318.2187
525 319.2260 318.2187
526 319.2260 318.2187
527 319.2262 318.2189
528 320.0216 319.0143
529 320.1935 319.1862
530 321.0643 320.0570
531 321.0665 320.0592
532 321.0932 320.0859
533 321.0933 320.0860
534 321.0942 320.0869
535 321.0948 320.0875
536 321.0950 320.0877
537 321.0956 320.0883
538 321.0956 320.0883
539 321.0958 320.0885
540 321.0958 320.0885
541 321.0961 320.0888
542 321.1291 320.1218
543 321.1307 320.1234
544 321.1312 320.1239
545 321.1313 320.1240
546 321.1313 320.1240
547 321.1313 320.1240
548 321.1320 320.1247
549 321.1322 320.1249
550 321.1324 320.1251
551 321.1324 320.1251
552 321.1324 320.1251
553 321.1324 320.1251
554 321.1324 320.1251
555 321.1325 320.1252
556 321.1326 320.1253
557 321.1326 320.1253
558 321.1326 320.1253
559 321.1326 320.1253
560 321.1326 320.1253
561 321.1326 320.1253
562 321.1326 320.1253
563 321.1327 320.1254
564 321.1566 320.1493
565 321.1687 320.1614
566 321.1687 320.1614
567 321.1688 320.1615
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