MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4247)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
368 313.1284 312.1211
369 313.1285 312.1212
370 313.1297 312.1224
371 313.1299 312.1226
372 313.1302 312.1229
373 313.1638 312.1565
374 313.1638 312.1565
375 313.1639 312.1566
376 313.1639 312.1566
377 313.1639 312.1566
378 313.1641 312.1568
379 313.1643 312.1570
380 313.1643 312.1570
381 313.1644 312.1571
382 313.1644 312.1571
383 313.1648 312.1575
384 313.1651 312.1578
385 313.1824 312.1751
386 313.1827 312.1754
387 313.1828 312.1755
388 313.1828 312.1755
389 313.1836 312.1763
390 313.1839 312.1766
391 313.1842 312.1769
392 313.1843 312.1770
393 313.1844 312.1771
394 313.1845 312.1772
395 313.2207 312.2134
396 313.2388 312.2315
397 313.2389 312.2316
398 313.2392 312.2319
399 314.0461 313.0388
400 314.0462 313.0389
401 314.1977 313.1904
402 315.0669 314.0596
403 315.0751 314.0678
404 315.0754 314.0681
405 315.0757 314.0684
406 315.0767 314.0694
407 315.1055 314.0982
408 315.1133 314.1060
409 315.1140 314.1067
410 315.1217 314.1144
411 315.1218 314.1145
412 315.1218 314.1145
413 315.1219 314.1146
414 315.1222 314.1149
415 315.1426 314.1353
416 315.1427 314.1354
417 315.1429 314.1356
418 315.1432 314.1359
419 315.1441 314.1368
420 315.1443 314.1370
421 315.1465 314.1392
422 315.1578 314.1505
423 315.1580 314.1507
424 315.1581 314.1508
425 315.1583 314.1510
426 315.1584 314.1511
427 315.1585 314.1512
428 315.1586 314.1513
429 315.1598 314.1525
430 315.1605 314.1532
431 316.0252 315.0179
432 317.0547 316.0474
433 317.0994 316.0921
434 317.0999 316.0926
435 317.1002 316.0929
436 317.1004 316.0931
437 317.1006 316.0933
438 317.1006 316.0933
439 317.1007 316.0934
440 317.1008 316.0935
441 317.1372 316.1299
442 317.1374 316.1301
443 317.1375 316.1302
444 317.1375 316.1302
445 317.1375 316.1302
446 317.1375 316.1302
447 317.1376 316.1303
448 317.1376 316.1303
449 317.1376 316.1303
450 317.1377 316.1304
451 317.1377 316.1304
452 317.1377 316.1304
453 317.1377 316.1304
454 317.1729 316.1656
455 317.1736 316.1663
456 317.1736 316.1663
457 317.1737 316.1664
458 317.1740 316.1667
459 317.1740 316.1667
460 317.1743 316.1670
461 317.1762 316.1689
462 317.2198 316.2125
463 317.2199 316.2126
464 318.0420 317.0347
465 318.1809 317.1736
466 318.1924 317.1851
467 318.1927 317.1854
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