MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4247)

Page: 1 2 3 4 5 6 … 43 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3710 465.2643 928.5140 928.5090 5.43 1 24 0.053 +1Score > 24 indicates identity RLQVQER + Deamidated (NQ)
3405 316.6826 631.3506 631.3475 4.91 0 24 0.068 +1Score > 25 indicates identity LCIAR
4038 652.3503 1302.6860 1302.6667 14.8 0 24 0.059 +1Score > 24 indicates identity QVVDTTVDLGQK + Deamidated (NQ)
3798 510.2820 1018.5494 1018.5447 4.65 1 23 0.066 +1Score > 23 indicates identity FASPNKNLK + Deamidated (NQ)
3661 444.2533 886.4920 886.4984 -7.21 1 21 0.11 +1Score > 25 indicates identity AVDALRSR
3675 450.7511 899.4876 899.4937 -6.71 1 21 0.066 +1Score > 22 indicates identity RGANVNLR + Deamidated (NQ)
3527 373.2173 744.4200 744.4130 9.48 0 21 0.15 +1Score > 25 indicates identity AGDLLTR
3383 308.6881 615.3616 615.3592 4.04 0 21 0.22 +1Score > 27 indicates identity ADIGIK
3608 416.2237 830.4328 830.4399 -8.49 0 20 0.099 +1Score > 24 indicates identity
Score > 23 indicates homology		 HDPHVVK
3546 382.2107 762.4068 762.4058 1.40 1 20 0.11 +1Score > 23 indicates identity MLEKAR + Oxidation (M)
3446 330.6786 659.3426 659.3391 5.39 0 20 0.2 +1Score > 25 indicates identity WVEAR
3633 429.7401 857.4656 857.4606 5.83 0 20 0.16 +1Score > 24 indicates identity IVNELNR + Deamidated (NQ)
3523 743.4447 742.4374 742.4337 4.97 0 19 0.14 +1Score > 23 indicates identity TGLSIPR
4057 331.6715 1322.6569 1322.6799 -17.4 1 19 0.15 +1Score > 24 indicates identity VSTGSLVMSMRR
3635 430.2505 858.4864 858.4811 6.27 0 19 0.17 +1Score > 24 indicates identity IAIVNSDK
3727 473.7480 945.4814 945.4879 -6.84 1 19 0.2 +1Score > 24 indicates identity ANQNRTLK + 2 Deamidated (NQ)
3767 494.7596 987.5046 987.4985 6.22 0 18 0.074 +1Score > 25 indicates identity
Score > 20 indicates homology		 LQALDGSQR + Deamidated (NQ)
3529 373.2285 744.4424 744.4494 -9.30 1 18 0.19 +1Score > 24 indicates identity RVLQTK + Deamidated (NQ)
3310 502.3262 501.3189 501.3275 -17.0 1 18 0.32 +1Score > 26 indicates identity GGKLK
3487 351.1843 700.3540 700.3616 -10.8 1 18 0.084 +1Score > 20 indicates identity RGQSPR + Deamidated (NQ)
3610 416.2468 830.4790 830.4749 4.98 0 18 0.12 +1Score > 22 indicates identity TIEQVLK + Deamidated (NQ)
3626 426.2190 850.4234 850.4297 -7.34 1 18 0.21 +1Score > 24 indicates identity YADQAKR
3766 494.7593 987.5040 987.4985 5.61 0 18 0.3 +1Score > 25 indicates identity LQALDGSQR + Deamidated (NQ)
3646 437.2581 872.5016 872.5079 -7.19 1 17 0.23 +1Score > 24 indicates identity IAKIASNR + Deamidated (NQ)
3833 525.7672 1049.5198 1049.5288 -8.49 1 17 0.27 +1Score > 24 indicates identity MEQTTRIR + Oxidation (M)
3312 503.2862 502.2789 502.2751 7.61 0 17 0.44 +1Score > 29 indicates identity
Score > 26 indicates homology		 ALDGK
3450 665.3539 664.3466 664.3432 5.20 0 17 0.15 +1Score > 22 indicates identity EIFEK
3971 406.8655 1217.5747 1217.5888 -11.6 1 17 0.25 +1Score > 24 indicates identity NKTNVEVDAAR + 2 Deamidated (NQ)
3606 415.2437 828.4728 828.4566 19.7 1 17 0.24 +1Score > 23 indicates identity NRAALER
3489 352.2046 702.3946 702.3912 4.92 0 17 0.42 +1Score > 26 indicates identity SLADAVK
3580 402.6898 803.3650 803.3524 15.8 0 17 0.18 +1Score > 22 indicates identity EYAFMK + Oxidation (M)
3644 436.7663 871.5180 871.5127 6.12 0 17 0.21 +1Score > 23 indicates identity SSIPITVR
3643 435.7765 869.5384 869.5334 5.79 0 17 0.067 +1Score > 18 indicates identity VALNILAR + Deamidated (NQ)
3367 586.3957 585.3884 585.3962 -13.3 1 17 0.19 +1Score > 22 indicates identity ALRVK
3340 529.3487 528.3414 528.3384 5.79 0 16 0.24 +1Score > 23 indicates identity AGILR
3652 439.7429 877.4712 877.4658 6.26 0 16 0.27 +1Score > 23 indicates identity LNTLFDR
3355 563.3334 562.3261 562.3227 6.05 0 16 0.22 +1Score > 22 indicates identity AGFLR
3545 381.2123 760.4100 760.4119 -2.47 0 16 0.1 +1Score > 26 indicates identity
Score > 19 indicates homology		 IEVWSK
3592 817.4462 816.4389 816.4341 5.89 0 16 0.29 +1Score > 25 indicates identity
Score > 23 indicates homology		 TADLLER
3424 323.2018 644.3890 644.3857 5.18 0 16 0.16 +1Score > 23 indicates identity
Score > 20 indicates homology		 LAVAGSK
3953 599.8158 1197.6170 1197.5990 15.1 0 16 0.23 +1Score > 22 indicates identity SSPISSSSPPPR
3973 609.8310 1217.6474 1217.6404 5.77 0 16 0.087 +1Score > 23 indicates identity
Score > 18 indicates homology		 IQLDPVTYNR
3526 373.2026 744.3906 744.4017 -14.9 0 16 0.56 +1Score > 26 indicates identity QIEVQK + Deamidated (NQ)
3659 443.7558 885.4970 885.4920 5.75 0 15 0.35 +1Score > 23 indicates identity IANNEVVK
3587 407.7452 813.4758 813.4708 6.17 0 15 0.057 +1Score > 23 indicates identity
Score > 16 indicates homology		 IVAELNR
3670 446.7630 891.5114 891.5065 5.50 0 15 0.15 +1Score > 19 indicates identity VLEYLQK
3461 337.2175 672.4204 672.4170 5.09 0 15 0.2 +1Score > 22 indicates identity
Score > 21 indicates homology		 VSIIGGK
3575 401.2346 800.4546 800.4504 5.30 1 15 0.26 -1Score > 25 indicates identity
Score > 22 indicates homology		 LAKANQR + Deamidated (NQ)
3595 410.7227 819.4308 819.4239 8.47 0 15 0.36 +1Score > 23 indicates identity VGINGFGR + Deamidated (NQ)
3913 572.2889 1142.5632 1142.5832 -17.5 1 15 0.26 +1Score > 22 indicates identity ADWRTQLPR + Deamidated (NQ)
3649 437.7560 873.4974 873.4807 19.1 0 15 0.13 +1Score > 24 indicates identity
Score > 19 indicates homology		 QVVESLAK + Deamidated (NQ)
3743 480.7796 959.5446 959.5287 16.6 0 15 0.32 +1Score > 22 indicates identity QQTLLTEK
3648 437.7496 873.4846 873.4807 4.49 0 14 0.18 +1Score > 25 indicates identity
Score > 19 indicates homology		 ISADIVEK
3382 308.1837 614.3528 614.3500 4.65 0 14 0.28 +1Score > 21 indicates identity GAQALR
3485 350.7230 699.4314 699.4279 5.05 0 14 0.3 +1Score > 21 indicates identity QNVLVK
3639 433.2453 864.4760 864.4705 6.39 0 14 0.21 +1Score > 20 indicates identity
Score > 20 indicates homology		 LVTTFQR + Deamidated (NQ)
4106 458.2502 1371.7288 1371.7245 3.08 0 14 0.17 +1Score > 23 indicates identity
Score > 19 indicates homology		 DTQSILPLSPSSK
4029 643.8516 1285.6886 1285.6779 8.37 0 14 0.07 +1Score > 23 indicates identity
Score > 15 indicates homology		 LVQSYHAVVDR
3820 348.8749 1043.6029 1043.5863 15.9 1 14 0.099 +1Score > 22 indicates identity
Score > 16 indicates homology		 TTPLEEVKK
3653 440.2485 878.4824 878.4749 8.59 0 14 0.15 +1Score > 21 indicates identity
Score > 18 indicates homology		 ELVLYNK + Deamidated (NQ)
3552 388.2158 774.4170 774.4123 6.10 0 14 0.33 +1Score > 25 indicates identity
Score > 21 indicates homology		 AVVEETK
3451 333.1809 664.3472 664.3432 6.14 0 14 0.36 +1Score > 22 indicates identity EIFEK
3458 672.4070 671.3997 671.3966 4.64 0 14 0.68 +1Score > 24 indicates identity LSSIPR
3899 563.3273 1124.6400 1124.6553 -13.6 0 13 0.19 +1Score > 19 indicates identity LLLDIAQSPR
3536 375.6817 749.3488 749.3378 14.8 0 13 0.41 +1Score > 23 indicates identity
Score > 22 indicates homology		 MEVQAR + Deamidated (NQ); Oxidation (M)
3354 562.2610 561.2537 561.2507 5.41 0 13 0.19 +1Score > 19 indicates identity SGDQR
3564 395.2228 788.4310 788.4181 16.5 0 13 0.35 +1Score > 24 indicates identity
Score > 21 indicates homology		 WLLDSR
3678 451.2431 900.4716 900.4665 5.76 0 13 0.53 +1Score > 23 indicates identity LQAALDNR + Deamidated (NQ)
3475 344.2133 686.4120 686.4075 6.60 0 13 0.18 +1Score > 23 indicates identity
Score > 18 indicates homology		 VLVSNR
3972 609.8234 1217.6322 1217.6186 11.2 1 13 0.63 +1Score > 24 indicates identity
Score > 24 indicates homology		 VQNALLSRCR + 2 Deamidated (NQ)
3696 458.7610 915.5074 915.5138 -6.90 1 13 0.74 +1Score > 24 indicates identity TRSNTLPK
4092 683.8578 1365.7010 1365.7074 -4.68 1 13 0.51 +1Score > 23 indicates identity
Score > 22 indicates homology		 SRYIMTTELPR
3904 565.3367 1128.6588 1128.6543 4.06 0 13 0.22 +1Score > 20 indicates identity
Score > 19 indicates homology		 WINTIQLIK + Deamidated (NQ)
3505 361.6974 721.3802 721.3680 17.0 0 13 0.48 +1Score > 22 indicates identity ETMTLK
3522 743.4436 742.4363 742.4337 3.49 0 13 0.62 +1Score > 24 indicates identity
Score > 23 indicates homology		 ALVTSPR
3821 522.8096 1043.6046 1043.5863 17.6 1 13 0.12 +1Score > 22 indicates identity
Score > 16 indicates homology		 TTPLEEVKK
3533 374.2314 746.4482 746.4439 5.83 0 13 0.2 +1Score > 18 indicates identity ALQLFR
3668 446.7161 891.4176 891.4086 10.1 0 13 0.33 +1Score > 23 indicates identity
Score > 20 indicates homology		 QTSQHYK + Deamidated (NQ)
3708 310.5119 928.5139 928.4978 17.4 0 13 0.72 +1Score > 24 indicates identity AEIAQIER
4043 437.2315 1308.6727 1308.6938 -16.2 0 13 0.17 +1Score > 22 indicates identity
Score > 17 indicates homology		 EHAISWGQLLR
3561 393.7297 785.4448 785.4395 6.75 0 13 0.41 +1Score > 22 indicates identity
Score > 21 indicates homology		 LGKPTDR
3758 488.7777 975.5408 975.5349 6.10 1 12 0.12 +1Score > 22 indicates identity
Score > 16 indicates homology		 ISSVAKDTR
3955 600.3427 1198.6708 1198.6921 -17.7 1 12 0.46 +1Score > 22 indicates identity KLVLEADGLNK
3735 317.8276 950.4610 950.4709 -10.4 0 12 0.24 +1Score > 23 indicates identity
Score > 19 indicates homology		 EQFQTLGK + Deamidated (NQ)
3341 531.3285 530.3212 530.3176 6.75 0 12 1 +1Score > 29 indicates identity
Score > 25 indicates homology		 AVVSR
3543 380.2364 758.4582 758.4538 5.91 1 12 0.55 +1Score > 22 indicates identity KLIQEK + Deamidated (NQ)
3464 338.1947 674.3748 674.3824 -11.1 1 12 0.24 +1Score > 22 indicates identity
Score > 18 indicates homology		 GRSISR
3528 745.4496 744.4423 744.4381 5.64 0 12 0.33 +1Score > 24 indicates identity
Score > 20 indicates homology		 LAATLEK
4008 632.2996 1262.5846 1262.5739 8.55 1 12 0.64 +1Score > 23 indicates identity
Score > 22 indicates homology		 NASGQDTGLKDR + 2 Deamidated (NQ)
4184 767.4041 1532.7936 1532.8021 -5.50 1 12 0.18 +1Score > 23 indicates identity
Score > 17 indicates homology		 FISQPGIMREDLK
3607 415.7421 829.4696 829.4545 18.2 0 12 0.81 +1Score > 25 indicates identity
Score > 23 indicates homology		 ADVADVLK
3584 403.7022 805.3898 805.3930 -3.88 0 12 0.82 +1Score > 23 indicates identity
Score > 23 indicates homology		 ASADSSLR
3992 416.5485 1246.6237 1246.6088 11.9 1 11 0.22 +1Score > 24 indicates identity
Score > 17 indicates homology		 MRLNNGLEQR + Deamidated (NQ); Oxidation (M)
3848 534.7855 1067.5564 1067.5764 -18.7 1 11 0.16 +1Score > 22 indicates identity
Score > 16 indicates homology		 RDYFQLVK
3946 594.3279 1186.6412 1186.6557 -12.2 1 11 0.22 +1Score > 24 indicates identity
Score > 17 indicates homology		 VSSQQPSKVVK + Deamidated (NQ)
3970 609.3253 1216.6360 1216.6412 -4.20 1 11 0.61 +1Score > 25 indicates identity
Score > 22 indicates homology		 DVRTSPSQSLK
3664 445.7349 889.4552 889.4505 5.34 0 11 0.71 +1Score > 25 indicates identity
Score > 22 indicates homology		 LSNPSSGTK
3496 716.4339 715.4266 715.4228 5.34 0 11 0.57 +1Score > 22 indicates identity
Score > 21 indicates homology		 NSLILR + Deamidated (NQ)
4058 662.3370 1322.6594 1322.6354 18.2 0 11 0.76 +1Score > 24 indicates identity
Score > 22 indicates homology		 QSIEDFVESIR + Deamidated (NQ)
3862 543.7670 1085.5194 1085.5175 1.75 0 11 0.49 +1Score > 22 indicates identity
Score > 21 indicates homology		 MTPSPQPQGK + Oxidation (M)
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