MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 46)

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+1

Accession Score Description
1 IGF1_BOVIN 698 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 480 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 195 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q2UU95_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 156 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 Q2U480_ASPOR 143 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+7

Accession Score Description
1 Q2U2R5_ASPOR 140 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

+8

Accession Score Description
1 Q2ULW0_ASPOR 100 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1

-9

Accession Score Description
1 Q2UK92_ASPOR 71 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1
Score Mass Matches Sequences emPAI
9.1 Q2UK92_ASPOR 71 63866 6 (3) 6 (3) 0.16
AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

-6 peptide matches (6 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3404   315.7021 629.3896 629.3860 5.75 1 3 3.4 +5Score > 23 indicates identity
Score > 21 indicates homology		 U R.VNIKR.E + Deamidated (NQ)
3690   457.2428 912.4710 912.4665 4.99 0 16 0.16 +1Score > 21 indicates identity U K.TQAPGGAPSK.A
3715   466.2686 930.5226 930.5175 5.58 0 32 0.0012 +1Score > 24 indicates identity
Score > 15 indicates homology		 U R.VPYLPSQK.T
4063   667.3497 1332.6848 1332.6786 4.69 0 40 0.0013 +1Score > 24 indicates identity U R.LGQLIGGAGNYDR.G
4135   715.3814 1428.7482 1428.7401 5.67 0 41 0.0012 +1Score > 24 indicates identity U R.YIPSVGQYLSFR.V
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_4.raw

Score > 24 indicates identity

Score > 17 indicates homology
4349   695.6831 2084.0275 2083.9963 15.0 0 4 1 -1Score > 24 indicates identity
Score > 17 indicates homology		 U R.VSAAWGEAGPISHQEEFLR.N + Deamidated (NQ)
No other peptide matches in query

+10

Accession Score Description
1 EF1A_ASPOR 51 Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1
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