MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (46 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 IGF1_BOVIN 698 42206 58 (37) 24 (19) 7.91 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal
2 FHP_YEAST 480 44846 32 (23) 16 (12) 3.14 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2
3 TRYP_PIG 195 25078 9 (8) 4 (4) 0.87 Trypsin OS=Sus scrofa PE=1 SV=1
4 Q2UU95_ASPOR 191 33878 10 (8) 9 (8) 1.11 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1
5 Q2UHF6_ASPOR 156 54290 12 (7) 12 (7) 0.51 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1
6 Q2U480_ASPOR 143 19581 11 (7) 11 (7) 2.04 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1
7 Q2U2R5_ASPOR 140 49765 13 (9) 13 (9) 0.78 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1
8 Q2ULW0_ASPOR 100 84939 9 (4) 9 (4) 0.16 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1
9 Q2UK92_ASPOR 71 63866 6 (3) 6 (3) 0.16 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1
10 EF1A_ASPOR 51 50492 4 (2) 4 (2) 0.13 Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1
11 Q2UV72_ASPOR 49 72822 1 (1) 1 (1) 0.05 FAD_binding_3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000018 PE=4 SV=1
12 Q2TWP0_ASPOR 41 55369 2 (2) 2 (2) 0.12 ATP synthase subunit beta OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090010000482 PE=3 SV=1
13 Q2U0N5_ASPOR 40 19103 1 (1) 1 (1) 0.18 GAT domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000365 PE=4 SV=1
14 Q2TZY8_ASPOR 39 29882 3 (2) 1 (1) 0.11 Exonuclease domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000664 PE=4 SV=1
15 Q2U287_ASPOR 38 59365 6 (1) 6 (1) 0.06 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1
16 Q2UCF4_ASPOR 34 34593 2 (1) 2 (1) 0.10 Ribosomal_L18_c domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090012000613 PE=3 SV=1
17 Q2U8D0_ASPOR 34 26124 1 (1) 1 (1) 0.13 CIA30 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000476 PE=4 SV=1
18 Q2UKH6_ASPOR 32 33420 2 (1) 2 (1) 0.10 60S acidic ribosomal protein P0 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000805 PE=3 SV=1
19 Q2U0S3_ASPOR 31 0 1 (1) 1 (1) 0.03 description
20 Q2UHE7_ASPOR 31 73756 1 (1) 1 (1) 0.04 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000477 PE=4 SV=1
21 ALF_ASPOR 31 39810 1 (1) 1 (1) 0.08 Fructose-bisphosphate aldolase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=fbaA PE=2 SV=2
22 Q2U385_ASPOR 29 32548 1 (1) 1 (1) 0.10 RelA_SpoT domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000141 PE=4 SV=1
23 Q2TVX9_ASPOR 28 84507 2 (1) 1 (1) 0.04 Actin binding protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=Aoabp1 PE=4 SV=1
24 Q2UK62_ASPOR 27 75289 1 (1) 1 (1) 0.04 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000939 PE=4 SV=1
25 Q2U724_ASPOR 26 62067 2 (1) 1 (1) 0.05 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090120000001 PE=4 SV=1
26 Q2UV77_ASPOR 26 0 3 (1) 3 (1) 0.07 description
27 Q2UGL9_ASPOR 25 58109 1 (1) 1 (1) 0.06 Mevalonate kinase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000793 PE=3 SV=1
28 ACLO_ASPOR 25 0 1 (1) 1 (1) 0.10 description
29 Q2U7W3_ASPOR 24 168122 2 (1) 2 (1) 0.02 PH domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000677 PE=4 SV=1
30 Q2U426_ASPOR 24 36607 2 (1) 2 (1) 0.09 Transaldolase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000520 PE=4 SV=1
31 Q2UHA9_ASPOR 23 39464 1 (1) 1 (1) 0.08 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000518 PE=4 SV=1
32 Q2URM8_ASPOR 22 17983 1 (1) 1 (1) 0.19 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000761 PE=3 SV=1
33 Q2U6V3_ASPOR 22 17333 1 (1) 1 (1) 0.20 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090120000082 PE=4 SV=1
34 Q2UQ81_ASPOR 21 82237 1 (1) 1 (1) 0.04 AP complex subunit beta OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001357 PE=3 SV=1
35 Q2UU27_ASPOR 21 68675 1 (1) 1 (1) 0.05 Aminotran_1_2 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000484 PE=4 SV=1
36 Q2U984_ASPOR 20 31060 1 (1) 1 (1) 0.11 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000133 PE=3 SV=1
37 Q2UTJ6_ASPOR 20 179297 3 (1) 3 (1) 0.02 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000707 PE=3 SV=1
38 Q2UK87_ASPOR 20 68239 1 (1) 1 (1) 0.05 F-box domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000911 PE=4 SV=1
39 Q2ULP0_ASPOR 18 0 1 (1) 1 (1) 0.07 description
40 Q2UFN5_ASPOR 17 138148 6 (1) 6 (1) 0.02 Structural maintenance of chromosomes protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090026000136 PE=3 SV=1
41 PEPA_ASPOR 17 0 1 (1) 1 (1) 0.07 description
42 Q2UT97_ASPOR 16 59238 1 (1) 1 (1) 0.06 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000104 PE=4 SV=1
43 Q2UKZ5_ASPOR 16 0 2 (1) 2 (1) 0.03 description
44 Q2U2P6_ASPOR 15 46059 1 (1) 1 (1) 0.07 Mediator of RNA polymerase II transcription subunit 16 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=MED16 PE=3 SV=1
45 Q2UM33_ASPOR 15 0 2 (1) 2 (1) 0.04 description
46 Q2URE4_ASPOR 15 57983 1 (1) 1 (1) 0.06 MFS domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000863 PE=4 SV=1

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