MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 46)

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+1

Accession Score Description
1 IGF1_BOVIN 698 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 480 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 195 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q2UU95_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 156 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 Q2U480_ASPOR 143 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+7

Accession Score Description
1 Q2U2R5_ASPOR 140 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

-8

Accession Score Description
1 Q2ULW0_ASPOR 100 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1
Score Mass Matches Sequences emPAI
8.1 Q2ULW0_ASPOR 100 84939 9 (4) 9 (4) 0.16
Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3419   322.1942 642.3738 642.3701 5.90 0 14 0.18 +1Score > 19 indicates identity U R.NLTPAK.T
3443   330.1907 658.3668 658.3650 2.87 0 12 1 +1Score > 26 indicates identity
Score > 25 indicates homology		 U R.ELAQAK.N
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_4.raw

Score > 20 indicates identity
3470   343.2043 684.3940 684.3919 3.20 0 40 0.00056 -1Score > 20 indicates identity U R.QPALTR.G
3.20 0 26 0.014 2 KPSPTR  
19.6 0 11 0.44 3 GPAAIQK   + Deamidated (NQ)
-13.2 1 11 0.46 4 KPQRR   + Deamidated (NQ)
-13.2 1 11 0.46 4 KPRQR   + Deamidated (NQ)
19.6 0 9 0.67 6 QPEALK  
19.6 0 9 0.67 6 QPELAK  
3.20 1 6 1.3 8 KVPADR  
3.20 0 3 2.8 9 AGATIPR  
3.20 1 3 2.8 9 KIGPDR  
3663   445.2451 888.4756 888.4705 5.79 0 5 1 +1Score > 24 indicates identity
Score > 17 indicates homology		 U K.QFPDLAAK.T
3815   519.2832 1036.5518 1036.5441 7.52 0 45 0.00024 +1Score > 22 indicates identity U K.ASVDEYILK.Q
3851   359.8509 1076.5309 1076.5250 5.42 1 23 0.079 +1Score > 24 indicates identity U K.YGPQEAEKR.E
3865   363.1985 1086.5737 1086.5920 -16.9 1 7 1.1 +3Score > 24 indicates identity
Score > 20 indicates homology		 U R.ELAQAKNLAK.A + 2 Deamidated (NQ)
4365   724.3676 2170.0810 2170.0542 12.3 0 28 0.0024 +1Score > 23 indicates identity
Score > 14 indicates homology		 U R.GVEIVHGDLNDEASLVEAFR.G + Deamidated (NQ)
4367   732.7181 2195.1325 2195.1011 14.3 0 40 0.00021 +1Score > 23 indicates identity
Score > 16 indicates homology		 U K.YAWVQPVGPSTPITTIGDHR.K + Deamidated (NQ)

+9

Accession Score Description
1 Q2UK92_ASPOR 71 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+10

Accession Score Description
1 EF1A_ASPOR 51 Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1
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