MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 46)

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+1

Accession Score Description
1 IGF1_BOVIN 698 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 480 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 195 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q2UU95_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 156 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 Q2U480_ASPOR 143 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

-7

Accession Score Description
1 Q2U2R5_ASPOR 140 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1
Score Mass Matches Sequences emPAI
7.1 Q2U2R5_ASPOR 140 49765 13 (9) 13 (9) 0.78
WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

-13 peptide matches (13 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3443   330.1907 658.3668 658.3650 2.87 0 12 1 +1Score > 26 indicates identity
Score > 25 indicates homology		 U K.IQAQAK.A + Deamidated (NQ)
3479   345.1974 688.3802 688.3756 6.80 0 17 0.38 +1Score > 25 indicates identity U K.GTVADVK.C
3482   350.2128 698.4110 698.4075 5.06 0 38 0.00093 +1Score > 20 indicates identity U R.GLAVSPR.H
3596   410.7454 819.4762 819.4715 5.80 0 26 0.012 +1Score > 20 indicates identity U R.NLAIHPR.E
3623   423.7361 845.4576 845.4494 9.72 0 25 0.048 +2Score > 25 indicates identity U R.LITGEADK.T
3842   533.2407 1064.4668 1064.4597 6.73 0 23 0.0089 +1Score > 20 indicates identity
Score > 15 indicates homology		 U K.CWDLETNK.V
3898   375.2191 1122.6355 1122.6298 5.03 0 40 0.00036 +1Score > 18 indicates identity U R.VISGHLGWVR.S
3902   376.8898 1127.6476 1127.6411 5.75 0 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology		 U R.SLIQRPSATR.Q
3947   396.9018 1187.6836 1187.6761 6.26 1 0 4.7 +2Score > 19 indicates identity U R.LITGEADKTIK.V
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_4.raw

Score > 19 indicates identity
3952   399.9055 1196.6947 1196.6877 5.80 0 21 0.037 -1Score > 19 indicates identity U R.LTLTGHISTVR.G
-6.91 0 6 0.99 2 NAAAALIWIVR  
4065   673.3394 1344.6642 1344.6561 6.03 0 42 0.00081 +1Score > 24 indicates identity U R.EFTFASASTGSIK.Q
4075   676.8036 1351.5926 1351.5867 4.41 0 40 0.00054 +1Score > 21 indicates identity
Score > 20 indicates homology		 U R.HPYLFSCGEDK.M
4321   960.9533 1919.8920 1919.8649 14.1 0 50 0.00014 +1Score > 24 indicates identity U R.SLAVEPNNEWFASGAGDR.T + Deamidated (NQ)

+8

Accession Score Description
1 Q2ULW0_ASPOR 100 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1

+9

Accession Score Description
1 Q2UK92_ASPOR 71 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+10

Accession Score Description
1 EF1A_ASPOR 51 Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1
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