MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 46)

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+1

Accession Score Description
1 IGF1_BOVIN 698 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 480 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 195 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q2UU95_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 156 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

-6

Accession Score Description
1 Q2U480_ASPOR 143 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1
Score Mass Matches Sequences emPAI
6.1 Q2U480_ASPOR 143 19581 11 (7) 11 (7) 2.04
eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

-11 peptide matches (11 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3369   300.1806 598.3466 598.3439 4.66 0 19 0.039 +1Score > 18 indicates identity U R.VVEPR.S
3402   315.2041 628.3936 628.3908 4.54 0 18 0.061 +1Score > 23 indicates identity
Score > 19 indicates homology		 U R.ILDIR.D
3577   401.7449 801.4752 801.4708 5.50 1 8 1.1 +3Score > 23 indicates identity
Score > 21 indicates homology		 U K.VIQSSRL.-
3638   433.2400 864.4654 864.4593 7.14 0 27 0.014 +1Score > 21 indicates identity U R.ELVVDYK.V
3691   457.7592 913.5038 913.4981 6.27 0 47 0.00011 +1Score > 22 indicates identity
Score > 20 indicates homology		 U R.ALVINDGGR.E
3858   542.2982 1082.5818 1082.5760 5.36 0 53 1.9e-005 +1Score > 21 indicates identity
Score > 19 indicates homology		 U R.YLGVDLFTR.Q
3916   382.5183 1144.5331 1144.5261 6.06 0 38 0.00096 +1Score > 22 indicates identity
Score > 20 indicates homology		 U K.AAFEDGHGSVR.A
3966   403.8848 1208.6326 1208.6262 5.28 0 39 0.00099 +1Score > 22 indicates identity U R.ISVSPQTGQHR.Y
4164   745.3826 1488.7506 1488.7395 7.49 0 17 0.079 +1Score > 24 indicates identity
Score > 18 indicates homology		 U R.SADTVPIPCNFIR.I
4169   750.4222 1498.8298 1498.8031 17.8 0 20 0.07 +1Score > 20 indicates identity U K.QGIPVVPQGELFSK.I + Deamidated (NQ)
4302   618.0307 1851.0703 1851.0400 16.3 0 33 0.0007 +1Score > 14 indicates identity U R.IGDILILQGRPCQVIR.I + Deamidated (NQ)

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
MMM1_ASPOR 19 0 6.1
description
2 samesets of MMM1_ASPOR
Q2PIY5_ASPOR 19 0
description
Q2UBR6_ASPOR 19 0
description

+7

Accession Score Description
1 Q2U2R5_ASPOR 140 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

+8

Accession Score Description
1 Q2ULW0_ASPOR 100 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1

+9

Accession Score Description
1 Q2UK92_ASPOR 71 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+10

Accession Score Description
1 EF1A_ASPOR 51 Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1
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