MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 46)

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+1

Accession Score Description
1 IGF1_BOVIN 698 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 480 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

+3

Accession Score Description
1 TRYP_PIG 195 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q2UU95_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1

-5

Accession Score Description
1 Q2UHF6_ASPOR 156 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1
Score Mass Matches Sequences emPAI
5.1 Q2UHF6_ASPOR 156 54290 12 (7) 12 (7) 0.51
Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

-12 peptide matches (12 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3464   338.1947 674.3748 674.3711 5.52 0 12 0.26 +2Score > 22 indicates identity
Score > 18 indicates homology		 U R.VSNSLR.A
3562   394.7134 787.4122 787.4188 -8.31 0 7 1 +2Score > 25 indicates identity
Score > 19 indicates homology		 U K.NVNTAIR.V + Deamidated (NQ)
3588   408.7478 815.4810 815.4753 7.09 0 51 8.3e-005 +1Score > 23 indicates identity U K.VTLELGGK.S
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_4.raw

Score > 23 indicates identity
3637   432.2374 862.4602 862.4548 6.26 0 37 0.002 -1Score > 23 indicates identity U K.LADLFER.D
6.26 0 10 1 2 GLQLFER   + Deamidated (NQ)
6.28 0 9 1.2 3 IAFLNQR   + 2 Deamidated (NQ)
6.26 0 3 5.4 4 LAEGQFAK  
3802   512.7432 1023.4718 1023.4661 5.58 0 29 0.0095 +1Score > 22 indicates identity U R.AAFEGEWSK.V
3840   530.7668 1059.5190 1059.5131 5.59 0 47 0.00026 +1Score > 24 indicates identity U K.GDVAAAAGCLR.Y
3897   561.3230 1120.6314 1120.6241 6.58 0 47 0.00012 +1Score > 20 indicates identity U K.IAFTGSTLVGR.M
3920   384.5547 1150.6423 1150.6346 6.66 0 18 0.095 +1Score > 20 indicates identity U R.ILVQEGIHDK.F
4055   661.4171 1320.8196 1320.8129 5.10 0 45 3.2e-005 +1Score > 13 indicates identity U K.IGPAIAAGNVVVLK.T
4143   725.3868 1448.7590 1448.7511 5.50 0 27 0.025 +1Score > 23 indicates identity U K.TAEQTPLSGLYAAK.L
4296   906.4776 1810.9406 1810.9141 14.6 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 U K.YEQPLGLFINNEFVK.A + Deamidated (NQ)
4444   1072.8633 3215.5681 3215.5357 10.1 0 6 0.61 +1Score > 23 indicates identity
Score > 16 indicates homology		 U R.TFETINPTNEKPIVAVQEADENDVEDAVK.A + Deamidated (NQ)

+6

Accession Score Description
1 Q2U480_ASPOR 143 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+7

Accession Score Description
1 Q2U2R5_ASPOR 140 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

+8

Accession Score Description
1 Q2ULW0_ASPOR 100 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1

+9

Accession Score Description
1 Q2UK92_ASPOR 71 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+10

Accession Score Description
1 EF1A_ASPOR 51 Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1
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