MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 46)


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+31

Accession Score Description
1 Q2UHA9_ASPOR 23 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000518 PE=4 SV=1

+32

Accession Score Description
1 Q2URM8_ASPOR 22 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000761 PE=3 SV=1

+33

Accession Score Description
1 Q2U6V3_ASPOR 22 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090120000082 PE=4 SV=1

+34

Accession Score Description
1 Q2UQ81_ASPOR 21 AP complex subunit beta OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001357 PE=3 SV=1

+35

Accession Score Description
1 Q2UU27_ASPOR 21 Aminotran_1_2 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000484 PE=4 SV=1

+36

Accession Score Description
1 Q2U984_ASPOR 20 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000133 PE=3 SV=1

+37

Accession Score Description
1 Q2UTJ6_ASPOR 20 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000707 PE=3 SV=1

+38

Accession Score Description
1 Q2UK87_ASPOR 20 F-box domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000911 PE=4 SV=1

+39

Accession Score Description
1 Q2ULP0_ASPOR 18 description

-40

Accession Score Description
1 Q2UFN5_ASPOR 17 Structural maintenance of chromosomes protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090026000136 PE=3 SV=1
Score Mass Matches Sequences emPAI
40.1 Q2UFN5_ASPOR 17 138148 6 (1) 6 (1) 0.02
Structural maintenance of chromosomes protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090026000136 PE=3 SV=1
3 samesets of Q2UFN5_ASPOR
Q2UAX0_ASPOR 17 0 2 (1) 2 (1) 0.12
description
Q2UAS1_ASPOR 17 0 1 (1) 1 (1) 0.05
description
Q2UTI9_ASPOR 17 0 1 (1) 1 (1) 0.12
description

-6 peptide matches (6 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3353   561.3289 560.3216 560.3282 -11.7 1 11 1.3 +5Score > 24 indicates identity U R.KNATK.N
3411   321.7202 641.4258 641.4224 5.34 0 17 0.052 +1Score > 16 indicates identity U R.LIIQR.K
3448   330.6833 659.3520 659.3490 4.65 0 5 1.1 +2Score > 27 indicates identity
Score > 18 indicates homology
U K.VNEGLK.K + Deamidated (NQ)
3797   509.7643 1017.5140 1017.5203 -6.14 1 0 4.1 +6Score > 25 indicates identity
Score > 19 indicates homology
U K.QRETLTNR.R + Deamidated (NQ)
3912   380.1933 1137.5581 1137.5414 14.6 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
U R.QYQELSSQR.S
4439   949.1221 2844.3445 2844.3388 1.99 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
U K.NDVMNLLESAGFSRSNPYYIVPQGR.V + 2 Deamidated (NQ); Oxidation (M)

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