MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 46)

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+21

Accession Score Description
1 ALF_ASPOR 31 Fructose-bisphosphate aldolase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=fbaA PE=2 SV=2

+22

Accession Score Description
1 Q2U385_ASPOR 29 RelA_SpoT domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000141 PE=4 SV=1

+23

Accession Score Description
1 Q2TVX9_ASPOR 28 Actin binding protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=Aoabp1 PE=4 SV=1

+24

Accession Score Description
1 Q2UK62_ASPOR 27 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000939 PE=4 SV=1

+25

Accession Score Description
1 Q2U724_ASPOR 26 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090120000001 PE=4 SV=1

+26

Accession Score Description
1 Q2UV77_ASPOR 26 description

+27

Accession Score Description
1 Q2UGL9_ASPOR 25 Mevalonate kinase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000793 PE=3 SV=1

+28

Accession Score Description
1 ACLO_ASPOR 25 description

+29

Accession Score Description
1 Q2U7W3_ASPOR 24 PH domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000677 PE=4 SV=1

-30

Accession Score Description
1 Q2U426_ASPOR 24 Transaldolase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000520 PE=4 SV=1
Score Mass Matches Sequences emPAI
30.1 Q2U426_ASPOR 24 36607 2 (1) 2 (1) 0.09
Transaldolase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000520 PE=4 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3557   393.2374 784.4602 784.4555 6.03 0 24 0.018 +1Score > 19 indicates identity U K.AAQVLQR.D
3650   438.7271 875.4396 875.4349 5.47 0 19 0.14 +1Score > 25 indicates identity
Score > 23 indicates homology		 U K.VSTEVDAR.L
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