MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1231_JBEI_4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:40:53 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,452

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 46)

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+1

Accession Score Description
1 IGF1_BOVIN 698 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

-2

Accession Score Description
1 FHP_YEAST 480 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2
Score Mass Matches Sequences emPAI
2.1 FHP_YEAST 480 44846 32 (23) 16 (12) 3.14
Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

-32 peptide matches (29 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3448   330.6833 659.3520 659.3490 4.62 0 5 1.1 +2Score > 27 indicates identity
Score > 18 indicates homology		 U K.VGDEIK.L
3467   680.3318 679.3245 679.3211 5.05 0 6 1 +1Score > 23 indicates identity
Score > 18 indicates homology		 U K.MSTVQV.- + Oxidation (M)
3911   568.7732 1135.5318 1135.5258 5.35 0 64 4.6e-006 +1Score > 24 indicates identity U R.QENQYDALR.H
3921   577.2725 1152.5304 1152.5233 6.16 0 69 9.4e-007 +1Score > 23 indicates identity
Score > 21 indicates homology		 U R.HYSLCSASTK.N
3963   602.3253 1202.6360 1202.6295 5.43 0 25 0.031 +1Score > 24 indicates identity
Score > 22 indicates homology		 U K.LSAPAGDFAINK.E
4165   749.8855 1497.7564 1497.7497 4.49 0 55 7.2e-006 +1Score > 23 indicates identity
Score > 16 indicates homology		 U K.NIDDLSVLMDHVK.Q
4166   500.2602 1497.7588 1497.7497 6.04 0 59 7.4e-006 +1Score > 23 indicates identity
Score > 20 indicates homology		 U K.NIDDLSVLMDHVK.Q
4178   757.8842 1513.7538 1513.7446 6.08 0 18 0.22 +1Score > 24 indicates identity U K.NIDDLSVLMDHVK.Q + Oxidation (M)
4179   505.5920 1513.7542 1513.7446 6.29 0 23 0.061 +1Score > 24 indicates identity U K.NIDDLSVLMDHVK.Q + Oxidation (M)
4217   532.2631 1593.7675 1593.7569 6.63 1 48 0.00023 +1Score > 24 indicates identity U R.HYSLCSASTKNGLR.F + Deamidated (NQ)
4219   798.4639 1594.9132 1594.9042 5.65 0 18 0.037 +1Score > 17 indicates identity U K.VGAQPNALATTVLAAAK.N
4220   532.6457 1594.9153 1594.9042 6.92 0 54 8.5e-006 +1Score > 16 indicates identity U K.VGAQPNALATTVLAAAK.N
4221   798.9554 1595.8962 1595.8882 5.01 0 20 0.036 +1Score > 18 indicates identity U K.VGAQPNALATTVLAAAK.N + Deamidated (NQ)
4222   798.9634 1595.9122 1595.8882 15.0 0 23 0.013 +1Score > 17 indicates identity U K.VGAQPNALATTVLAAAK.N + Deamidated (NQ)
4223 +1 535.2783 1602.8131 1602.8042 5.55 0 47 0.00026 +1Score > 24 indicates identity U R.ENFPAGLVSEYLHK.D
4224   802.4149 1602.8152 1602.8042 6.90 0 25 0.012 +1Score > 24 indicates identity
Score > 18 indicates homology		 U R.ENFPAGLVSEYLHK.D
4230   806.4609 1610.9072 1610.8992 5.01 0 52 2.5e-005 +1Score > 19 indicates identity U K.ATVPVLEQQGTVITR.T
4231   806.8892 1611.7638 1611.7563 4.71 0 21 0.1 +1Score > 23 indicates identity U K.HVDELLAECANVDK.I
4232   538.2625 1611.7657 1611.7563 5.84 0 45 0.00042 +1Score > 23 indicates identity U K.HVDELLAECANVDK.I
4233   538.3115 1611.9127 1611.8832 18.3 0 52 2e-005 +1Score > 18 indicates identity U K.ATVPVLEQQGTVITR.T + Deamidated (NQ)
4270   575.6450 1723.9132 1723.9032 5.76 0 24 0.0061 +1Score > 21 indicates identity
Score > 14 indicates homology		 U K.EYVASDIVEFTVKPK.F
4299   615.6528 1843.9366 1843.9250 6.26 0 21 0.028 +1Score > 23 indicates identity
Score > 18 indicates homology		 U K.NMLTEHTELLNIFNR.T
4306   620.9837 1859.9293 1859.9200 5.01 0 61 4.3e-006 +1Score > 24 indicates identity
Score > 19 indicates homology		 U K.NMLTEHTELLNIFNR.T + Oxidation (M)
4308   621.3183 1860.9331 1860.9040 15.6 0 19 0.034 +1Score > 24 indicates identity
Score > 17 indicates homology		 U K.NMLTEHTELLNIFNR.T + Deamidated (NQ); Oxidation (M)
4317   632.3632 1894.0678 1894.0564 6.01 0 17 0.057 +1Score > 17 indicates identity U K.IIVHTDTEPLINAAFLK.E
4351   704.4053 2110.1941 2110.1826 5.43 0 13 0.092 +1Score > 16 indicates identity U R.ALQIKPEHYPIVGEYLLK.A
4352   704.7398 2111.1976 2111.1666 14.7 0 29 0.0023 +1Score > 16 indicates identity U R.ALQIKPEHYPIVGEYLLK.A + Deamidated (NQ)
4354 +2 528.8069 2111.1985 2111.1666 15.1 0 25 0.0064 +1Score > 15 indicates identity U R.ALQIKPEHYPIVGEYLLK.A + Deamidated (NQ)
4364   724.3431 2170.0075 2169.9789 13.2 0 12 0.14 +1Score > 23 indicates identity
Score > 15 indicates homology		 U K.CNPNRPIYWIQSSYDEK.T + Deamidated (NQ)

+3

Accession Score Description
1 TRYP_PIG 195 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q2UU95_ASPOR 191 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 156 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 Q2U480_ASPOR 143 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+7

Accession Score Description
1 Q2U2R5_ASPOR 140 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

+8

Accession Score Description
1 Q2ULW0_ASPOR 100 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1

+9

Accession Score Description
1 Q2UK92_ASPOR 71 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+10

Accession Score Description
1 EF1A_ASPOR 51 Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1
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