MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 4 |
| MS data file | : | PRT1231_JBEI_4.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 1 May 2025 at 20:40:53 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,452 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 22 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IGF1_BOVIN | 698 | Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FHP_YEAST | 480 | Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 195 | Trypsin OS=Sus scrofa PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UU95_ASPOR | 191 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000405 PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UHF6_ASPOR | 156 | Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U480_ASPOR | 143 | eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U2R5_ASPOR | 140 | WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2ULW0_ASPOR | 100 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UK92_ASPOR | 71 | AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EF1A_ASPOR | 51 | Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 10.1 | EF1A_ASPOR |
51 | 50492 | 4 (2) | 4 (2) | 0.13 | |
| Elongation factor 1-alpha OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=tef1 PE=3 SV=1 | |||||||
4 peptide matches (4 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3544 |
380.7159 | 759.4172 | 759.4127 | 6.04 | 0 | 16 | 0.26 | 1Score > 25 indicates identity Score > 23 indicates homology |
U | K.SGDAAIVK.M | |
| 3583 |
403.1870 | 804.3594 | 804.3548 | 5.73 | 0 | 11 | 0.23 | 1Score > 22 indicates identity Score > 17 indicates homology |
U | K.CGGIDQR.T | |
| 3674 |
449.7922 | 897.5698 | 897.5647 | 5.70 | 0 | 30 | 0.002 | 1Score > 15 indicates identity |
U | R.QLIVALNK.M | |
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_4.rawScore > 23 indicates identity Score > 15 indicates homology |
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| 4077 |
452.9048 | 1355.6926 | 1355.6834 | 6.79 | 0 | 36 | 0.0005 | 1Score > 23 indicates identity Score > 15 indicates homology |
U | K.YEVTVIDAPGHR.D | |
| 15.1 | 1 | 1 | 1.7 | 2 | ELGYNKDQVYK | ||||||
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